Chen Chung-An, Wang Chun-Chi, Chen Yen-Ling, Jong Yuh-Jyh, Wu Shou-Mei
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chem Commun (Camb). 2014 Jul 28;50(59):8069-72. doi: 10.1039/c4cc03472d.
A "turn on/off" biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a "scorpion primer". This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.
