Singh Padmanabh, Thakur Mahendra K
Biochemistry and Molecular Biology Laboratory, Department of Zoology, Brain Research Centre, Banaras Hindu University, Varanasi, 221 005, India.
Biogerontology. 2014 Aug;15(4):339-46. doi: 10.1007/s10522-014-9504-5. Epub 2014 Jun 13.
Chromatin modifying enzymes DNA methyltransferases (DNMTs), histone deacetylase (HDAC) 2 and CREB binding protein (CBP) play a crucial role in memory, particularly during consolidation process which declines with advancing age. However, the expression of these enzymes and their effect on memory consolidation during aging are not clearly understood. In the present study, novel object recognition test was used to assess the memory consolidation followed by expression analysis of DNMTs, HDAC2 and CBP in the cerebral cortex and hippocampus of young, adult and old male mice. Object recognition memory was reduced in old as compared to young and adult. DNMT1 protein expression was high in the cerebral cortex and hippocampus of young male mice, but declined gradually with age. On the other hand, HDAC2 mRNA and protein expression increased in the hippocampus of old male mice as compared to young and adult. Alteration in the expression of these enzymes is correlated with reduced recognition memory in old.
染色质修饰酶DNA甲基转移酶（DNMTs）、组蛋白脱乙酰酶（HDAC）2和CREB结合蛋白（CBP）在记忆中起着至关重要的作用，尤其是在随着年龄增长而衰退的巩固过程中。然而，这些酶在衰老过程中的表达及其对记忆巩固的影响尚不清楚。在本研究中，使用新颖物体识别测试来评估记忆巩固，随后对年轻、成年和老年雄性小鼠大脑皮层和海马体中的DNMTs、HDAC2和CBP进行表达分析。与年轻和成年小鼠相比，老年小鼠的物体识别记忆降低。年轻雄性小鼠大脑皮层和海马体中DNMT1蛋白表达较高，但随着年龄增长逐渐下降。另一方面，与年轻和成年小鼠相比，老年雄性小鼠海马体中HDAC2 mRNA和蛋白表达增加。这些酶表达的改变与老年小鼠识别记忆降低相关。
