Mallett Christiane L, Lim Heeseung, Thind Kundan, Chen Yuhua, Ribot Emeline J, Martinez Francisco, Scholl Timothy J, Foster Paula J
Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada; Department of Medical Biophysics, Western University, London, Ontario, Canada.
J Magn Reson Imaging. 2014 Oct;40(4):848-56. doi: 10.1002/jmri.24433. Epub 2013 Nov 4.
To assess anatomic and functional magnetic resonance imaging (MRI) for monitoring of tumor volume and metabolism of orthotopic xenograft prostate cancer tumors.
Human-derived PC-3M cells were implanted into the prostate in 22 nude mice. Tumor volume and MRI appearance were monitored for up to 29 days. Histology was performed to detect metastases. Hyperpolarized [1-(13) C]pyruvate MRI was used to measure tumor metabolism on day 22.
Tumors were visible by MRI 9 days after tumor cell implantation. Tumor volume increased to 720 ± 190 mm(3) on day 29 of imaging. Metastasis was seen in the iliac lymph nodes at all timepoints, and in more distant lymph nodes at later timepoints, but was not detectable by MRI. Regions with low pyruvate uptake corresponded to regions with necrosis and had a higher lactate/pyruvate ratio (0.98 ± 0.4 vs. 1.6 ± 1.1).
MRI using the balanced steady-state free precession (bSSFP) sequence can be used to monitor tumor growth in orthotopic PC-3M tumors as early as 9 days post-injection. Hyperpolarized pyruvate MRI has potential to assess tumor metabolism and necrosis.
评估解剖学和功能磁共振成像（MRI）用于监测原位异种移植前列腺癌肿瘤的体积和代谢情况。
将人源PC-3M细胞植入22只裸鼠的前列腺中。对肿瘤体积和MRI表现进行长达29天的监测。进行组织学检查以检测转移情况。在第22天使用超极化[1-(13)C]丙酮酸MRI测量肿瘤代谢。
肿瘤细胞植入后9天，MRI可见肿瘤。成像第29天时，肿瘤体积增加至720±190立方毫米。在所有时间点均可见髂淋巴结转移，在后期时间点可见更远端淋巴结转移，但MRI无法检测到。丙酮酸摄取低的区域对应于坏死区域，且乳酸/丙酮酸比值更高（0.98±0.4对1.6±1.1）。
使用平衡稳态自由进动（bSSFP）序列的MRI可在注射后9天早期用于监测原位PC-3M肿瘤的生长。超极化丙酮酸MRI有潜力评估肿瘤代谢和坏死情况。
