[经寡脱氧核苷酸和CA125致敏的树突状细胞对裸鼠人卵巢癌OVCAR3异种移植瘤的免疫抑制作用]

[Immunosuppressive action of dendritic cells sensitized by oligodeoxynucleotides and CA125 on human OVCAR3 ovarian carcinoma xenografts in nude mice].

作者信息

Ye Mingzhu, Li Na, Huang Xiumin, Wang Daying, Shang Jing

机构信息

Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China. Email:

Department of Obstetrics & Gynecology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2014 Apr 22;94(15):1193-5.

PMID:24924723

Abstract

OBJECTIVE

To explore the immunosuppressive action of dendritic cells (DC) sensitized by oligodeoxynucleotides containing "un-methylated cytosine phosphodiester bond-guanylic acid" motif (CpG ODN) and CA125 on human ovarian carcinoma xenografts in nude mice.

METHODS

Human peripheral blood-derived dendritic cells were isolated and identified by flow cytometry. The DC sensitized by CpG ODN and CA125 were then co-cultured with T cells and finally cytotoxic T lymphocytes (CTL) were induced. Nude mice bearing OVCAR3 transplanted tumor were immunized with induced CTL by subcutaneous injection and tumor growth and cell apoptosis observed.

RESULTS

Premature DC had a low expression of CD83 and CD86. In vivo, delayed growth of OVCAR3 xenografts was observed after immunotherapy with CTL induced by DC pulsed by CpG ODN and CA125. The inhibition rate of tumor was 50.71% and it was better than CpG ODN-pulsed and CA125-pulsed groups (P < 0.05). Cell apoptotic rate was (29.6 ± 3.0)% in CpG ODN+CA125-pulsed group versus (21.8 ± 2.7)% in CpG ODN-pulsed group. And both were more than those of unpulsed and CA125-pulsed groups (P < 0.05).

CONCLUSION

CTL induced by DC sensitized by CpG ODN and CA125 can inhibit the growth of ovarian carcinoma xenografts and promote tumor cell apoptosis.

摘要

目的

探讨含“非甲基化胞嘧啶磷酸二酯键 - 鸟苷酸”基序的寡脱氧核苷酸（CpG ODN）致敏的树突状细胞（DC）与CA125对裸鼠人卵巢癌移植瘤的免疫抑制作用。

方法

分离人外周血来源的树突状细胞并通过流式细胞术进行鉴定。然后将经CpG ODN和CA125致敏的DC与T细胞共培养，最终诱导产生细胞毒性T淋巴细胞（CTL）。将携带OVCAR3移植瘤的裸鼠通过皮下注射用诱导产生的CTL进行免疫，观察肿瘤生长及细胞凋亡情况。

结果

未成熟DC的CD83和CD86表达较低。在体内，用经CpG ODN和CA125脉冲处理的DC诱导产生的CTL进行免疫治疗后，观察到OVCAR3异种移植瘤生长延迟。肿瘤抑制率为50.71%，优于CpG ODN脉冲组和CA125脉冲组（P < 0.05）。CpG ODN + CA125脉冲组细胞凋亡率为（29.6 ± 3.0）%，CpG ODN脉冲组为（21.8 ± 2.7）%。两者均高于未脉冲组和CA125脉冲组（P < 0.05）。