Huang Tzu-Lun, Huang Sun-Ping, Chang Chung-Hsing, Lin Kung-Hung, Sheu Min-Muh, Tsai Rong-Kung
Department of Ophthalmology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
Department of Molecular and Human Genetics, Tzu Chi University, Hualien, Taiwan.
Kaohsiung J Med Sci. 2014 Jul;30(7):331-6. doi: 10.1016/j.kjms.2014.02.009. Epub 2014 Apr 3.
To investigate the effects of cerebrolysin (Cbl) on optic nerves (ON) and retinal ganglion cells (RGC) in a rat model of ON crush. Rats received intravitreal injection of Cbl (n = 20), intra-ON injection of Cbl (n = 20), intraperitoneal injection (IPI) of Cbl (n = 20), or phosphate buffered saline (PBS; n = 20) every day for 2 weeks after ON crush injury. At 3 weeks post-trauma, RGC density was counted by retrograde labeling with FluoroGold and visual function was assessed by flash visual-evoked potentials. Activities of microglia after insults were quantified by immunohistochemical analysis of the presence of ED1 in the optic nerve. At 3 weeks postcrush, the densities of RGCs in the Cbl-IVI group (1125 ± 166/mm(2)) and in the Cbl-IPI treatment group (1328 ± 119/mm(2)) were significantly higher than those in the PBS group (641 ± 214/mm(2)). The flash visual-evoked potential measurements showed that latency of the P1 wave was significantly shorter in the Cbl-IVI- and Cbl-IPI-treated groups (105 ± 4 ms and 118 ± 26 ms, respectively) than in the PBS-treated group (170 ± 20 ms). However, only Cbl IPI treatment resulted in a significant decrease in the number of ED1-positive cells at the lesion sites of the ON (5 ± 2 cells/vs. 30 ± 4 cells/high-power field in control eyes). Treatment with intra-ON injection of Cbl was harmful to the optic nerve in the crush model. Systemic administration of Cbl had neuroprotective effects on RGC survival and visual function in the optic nerve crush model.
为研究脑蛋白水解物（Cbl）对大鼠视神经挤压伤模型中视神经（ON）和视网膜神经节细胞（RGC）的影响。视神经挤压伤后，大鼠连续2周每天接受玻璃体内注射Cbl（n = 20）、视神经内注射Cbl（n = 20）、腹腔注射（IPI）Cbl（n = 20）或磷酸盐缓冲盐水（PBS；n = 20）。创伤后3周，通过FluoroGold逆行标记计数RGC密度，并用闪光视觉诱发电位评估视觉功能。通过对视神经中ED1存在情况的免疫组织化学分析定量损伤后小胶质细胞的活性。挤压伤后3周，Cbl-IVI组（1125±166/mm²）和Cbl-IPI治疗组（1328±119/mm²）的RGC密度显著高于PBS组（641±214/mm²）。闪光视觉诱发电位测量显示，Cbl-IVI和Cbl-IPI治疗组的P1波潜伏期（分别为105±4 ms和118±26 ms）显著短于PBS治疗组（170±20 ms）。然而，只有Cbl IPI治疗导致视神经损伤部位ED1阳性细胞数量显著减少（5±2个细胞/高倍视野 vs. 对照眼中30±4个细胞/高倍视野）。在挤压伤模型中，视神经内注射Cbl对神经有害。在视神经挤压伤模型中，全身性给予Cbl对RGC存活和视觉功能具有神经保护作用。
