Dalila Adnan Siti, Mohd Said Mohd Shahrir, Shaharir Syahrul Sazliyana, Asrul Abdul Wahab, Low Soo Fin, Shamsul Azhar Shah, Sakthiswary Rajalingham
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Department of Microbiology and Immunology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Kaohsiung J Med Sci. 2014 Jul;30(7):337-42. doi: 10.1016/j.kjms.2014.02.010. Epub 2014 Apr 2.
The purpose of this study was to compare the serum interleukin (IL)-23 levels between rheumatoid arthritis (RA) patients and healthy controls and to determine the correlation of IL-23 levels with disease activity, joint damage and functional disability in RA. Serum samples were obtained from 45 patients with RA and 45 healthy controls. The enzyme-linked immunosorbent assay method was used for quantitative analysis of IL-23. All the RA patients were assessed for disease activity based on the 28-joint disease activity score, joint damage based on modified Sharp score, and functional ability using the Health Assessment Questionnaire-Disability Index. The mean serum IL-23 level was much higher among the RA patients (24.50 ± 13.98 pg/mL) compared to the controls (5.98 ± 3.40 pg/mL; p < 0.01). There was a significant positive relationship between IL-23 levels and disease activity and questionnaire scores (p = 0.003 and 0.020, respectively). On logistic regression analysis, IL-23 levels were significantly higher in patients with moderate to high disease activity (p = 0.008, odds ratio = 1.073, 95% confidence interval = 1.019-1.130) and patients with significant functional disability (p = 0.008, odds ratio = 1.085, 95% confidence interval = 1.021-1.153). RA patients have significantly higher levels of serum IL-23. The IL-23 levels correlate well with disease activity and functional disability but not with radiographic joint damage.
本研究的目的是比较类风湿关节炎（RA）患者与健康对照者的血清白细胞介素（IL）-23水平，并确定IL-23水平与RA患者疾病活动度、关节损伤及功能残疾之间的相关性。收集了45例RA患者和45例健康对照者的血清样本。采用酶联免疫吸附测定法对IL-23进行定量分析。所有RA患者均根据28关节疾病活动评分评估疾病活动度，根据改良Sharp评分评估关节损伤，并使用健康评估问卷残疾指数评估功能能力。与对照组（5.98±3.40 pg/mL；p<0.01）相比，RA患者的血清IL-23平均水平（24.50±13.98 pg/mL）要高得多。IL-23水平与疾病活动度和问卷评分之间存在显著正相关（分别为p = 0.003和0.020）。经逻辑回归分析，中度至高度疾病活动患者（p = 0.008，比值比 = 1.073，95%置信区间 = 1.019 - 1.130）和有显著功能残疾的患者（p = 0.008，比值比 = 1.085，95%置信区间 = 1.021 - 1.153）的IL-23水平显著更高。RA患者的血清IL-23水平显著更高。IL-23水平与疾病活动度和功能残疾密切相关，但与影像学关节损伤无关。
