Sato Kiminori, Kurita Takashi, Chitose Shun-Ichi, Umeno Hirohito, Nakashima Tadashi
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan.
Laryngoscope. 2014 Nov;124(11):2551-4. doi: 10.1002/lary.24776. Epub 2014 Jun 13.
OBJECTIVES/HYPOTHESIS: Vocal fold stellate cells (VFSCs) in the human maculae flavae located at both ends of the vocal fold mucosa are inferred to be involved in the metabolism of extracellular matrices of the vocal fold mucosa. Tension caused by phonation (vocal fold vibration) likely regulates the behavior of the VFSCs in the human maculae flava. Tensile and compressive strains have direct effects on cell morphology and structure, including changes in cytoskeletal structure and organization. Cytoskeletons play a role as mechanoreceptors for the cells. The microstructure of the intermediate filaments and the expression of their characteristic proteins were investigated regarding the human newborn VFSCs.
Histopathologic analysis of the human newborn vocal fold.
Three newborn vocal fold mucosae were investigated by immunohistochemistry and electron microscopy.
The intermediate filaments in the cytoplasm of the newborn VFSCs were few in number. However, their characteristic proteins (vimentin, desmin, GFAP [Glial fibrillary acidic protein], cytokeratin) had already expressed.
The function and fate of VFSCs are regulated by various microenvironmental factors. Not only chemical factors but also mechanical factors could also modulate VFSC behaviors. The cytoskeletal structure of the newborn VFSCs is under development. And the newborn VFSCs have not acquired mechanical regulation.
N/A.
目的/假设：位于声带黏膜两端的人黄斑部声带星状细胞（VFSCs）被推断参与声带黏膜细胞外基质的代谢。发声（声带振动）引起的张力可能调节人黄斑部VFSCs的行为。拉伸和压缩应变对细胞形态和结构有直接影响，包括细胞骨架结构和组织的变化。细胞骨架作为细胞的机械感受器发挥作用。研究了人类新生儿VFSCs中间丝的微观结构及其特征性蛋白的表达。
对人类新生儿声带进行组织病理学分析。
通过免疫组织化学和电子显微镜对三个新生儿声带黏膜进行研究。
新生儿VFSCs细胞质中的中间丝数量较少。然而，它们的特征性蛋白（波形蛋白、结蛋白、胶质纤维酸性蛋白[GFAP]、细胞角蛋白）已经表达。
VFSCs的功能和命运受多种微环境因素调节。不仅化学因素，机械因素也可调节VFSCs的行为。新生儿VFSCs的细胞骨架结构正在发育中。并且新生儿VFSCs尚未获得机械调节。
无。
