Ossetrova Natalia I, Sandgren David J, Blakely William F
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5693, USA
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5693, USA.
Radiat Prot Dosimetry. 2014 Jun;159(1-4):61-76. doi: 10.1093/rpd/ncu165. Epub 2014 Jun 12.
Development and validation of early-response radiation injury biomarkers are critical for effective triage and medical management of irradiated individuals. Plasma protein and haematological profiles were evaluated using multivariate linear-regression analysis to provide dose-response calibration curves for photon-radiation dose assessment in 30 rhesus macaques total-body-irradiated to 1-8.5 Gy with (60)Co gamma rays (0.55 Gy min(-1)). Equations for radiation dose received were established based on different combinations of protein biomarkers [i.e. C-reactive protein (CRP), serum amyloid A (SAA), interleukin 6 (IL-6) and Flt3 Ligand (Flt3L)] at samples collection time-points 6 h, 1, 2, 3, 4 and 7 d post-total-body irradiation. Dynamic changes in the levels of CRP, SAA, IL-6 and Flt3L may function as prognostic indicators of the time course and severity of acute radiation sickness (ARS). The combination of protein biomarkers provides greater accuracy for early radiation assessment than any one biomarker alone.
开发和验证早期反应性辐射损伤生物标志物对于受辐照个体的有效分诊和医疗管理至关重要。使用多元线性回归分析评估血浆蛋白和血液学特征，以提供30只恒河猴全身接受1 - 8.5 Gy（60）Coγ射线（0.55 Gy min⁻¹）照射的光子辐射剂量评估的剂量反应校准曲线。基于蛋白质生物标志物[即C反应蛋白（CRP）、血清淀粉样蛋白A（SAA）、白细胞介素6（IL - 6）和Flt3配体（Flt3L）]在全身照射后6小时、1天、2天、3天、4天和7天样本采集时间点的不同组合，建立了所接受辐射剂量的方程。CRP、SAA、IL - 6和Flt3L水平的动态变化可能作为急性放射病（ARS）病程和严重程度的预后指标。与任何单一生物标志物相比，蛋白质生物标志物的组合为早期辐射评估提供了更高的准确性。
