Rot A, Henderson L E, Sowder R, Leonard E J
Immunopathology Section, National Cancer Institute, Frederick, Maryland 21701.
J Leukoc Biol. 1989 Feb;45(2):114-20. doi: 10.1002/jlb.45.2.114.
A chemotactic tetrapeptide from culture fluids of Staphylococcus aureus was purified to homogeneity by reverse-phase high-pressure liquid chromatography. The peptide comprises equimolar methionine, leucine, isoleucine, and phenylalanine. It inhibited binding of fluoresceinated fMet-Leu-Phe-Lys to human monocytes, which showed that it interacted with the formyl-methionyl peptide receptor and suggested that it was a formyl-methionyl peptide. Based on a comparison of dose-response curves for inhibition of fluoresceinated fMet-Leu-Phe-Lys binding, the relative affinity of the peptide for the receptor was comparable to that of fMet-Leu-Phe-Lys. At optimal concentrations, chemotactic efficacy (percentage of monocytes migrating to the attractant) was 53 +/- 4%, in contrast to 36 +/- 3% for the reference attractant fMet-Leu-Phe. Since approximately 60% of human monocytes have formyl-peptide receptors, the bacterial peptide is capable of attracting all receptor-bearing monocytes.
通过反相高压液相色谱法将来自金黄色葡萄球菌培养液的一种趋化四肽纯化至同质。该肽由等摩尔的蛋氨酸、亮氨酸、异亮氨酸和苯丙氨酸组成。它抑制了荧光素标记的fMet-Leu-Phe-Lys与人单核细胞的结合，这表明它与甲酰甲硫氨酰肽受体相互作用，并提示它是一种甲酰甲硫氨酰肽。基于对抑制荧光素标记的fMet-Leu-Phe-Lys结合的剂量反应曲线的比较，该肽对受体的相对亲和力与fMet-Leu-Phe-Lys相当。在最佳浓度下，趋化效力（单核细胞迁移至趋化剂的百分比）为53±4%，而参考趋化剂fMet-Leu-Phe为36±3%。由于大约60%的人单核细胞具有甲酰肽受体，该细菌肽能够吸引所有带有受体的单核细胞。
