Valente Mariana, Nascimento Diana Santos, Cumano Ana, Pinto-do-Ó Perpétua
1 Stem-Cell Microenvironments in Repair/Regeneration Team, Microenvironments for NewTherapies Group, INEB-Instituto Nacional de Engenharia Biomédica, Universidade do Porto , Porto, Portugal .
Stem Cells Dev. 2014 Oct 1;23(19):2263-73. doi: 10.1089/scd.2014.0197. Epub 2014 Jul 14.
The identification, in the adult, of cardiomyocyte turnover events and of cardiac progenitor cells (CPCs) has revolutionized the field of cardiovascular medicine. However, the low rate of CPCs differentiation events reported both in vitro and in vivo, even after injury, raised concerns on the biological significance of these subsets. In this Comprehensive Review, we discuss the current understanding of cardiac Lin(-)Sca-1(+) cells in light of what is also known for cellular compartments with similar phenotypes in other organs. The Lin(-)Sca-1(+) heart subset is heterogeneous and displays a mesenchymal profile, characterized by a limited ability to generate cardiomyocytes in vitro and in vivo, even after injury. There is no evidence for Sca-1 expression in embryonic cardiovascular progenitors. In other organs, Sca-1 expression is mainly observed on mesoderm-derived cells, although it is not restricted to stem/progenitor cell populations. It is urgent to determine, at a single cell level, to which extent cardiac Lin(-)Sca-1(+) cells overlap with the fibroblast compartment.
在成体中对心肌细胞更新事件和心脏祖细胞（CPCs）的识别，彻底改变了心血管医学领域。然而，无论是在体外还是体内，即使在损伤后，所报道的CPCs分化事件发生率都很低，这引发了人们对这些细胞亚群生物学意义的担忧。在这篇综述中，我们根据在其他器官中具有相似表型的细胞区室的已知情况，讨论对心脏Lin(-)Sca-1(+)细胞的当前认识。心脏Lin(-)Sca-1(+)细胞亚群是异质性的，呈现间充质特征，其特点是即使在损伤后，在体外和体内产生心肌细胞的能力也有限。在胚胎心血管祖细胞中没有Sca-1表达的证据。在其他器官中，虽然Sca-1表达不限于干/祖细胞群体，但主要在中胚层来源的细胞上观察到。迫切需要在单细胞水平上确定心脏Lin(-)Sca-1(+)细胞与成纤维细胞区室的重叠程度。
