Bhattacharjee Ujjal, Beck Christie, Winter Arthur, Wells Carson, Petrich Jacob W
Department of Chemistry, Iowa State University , Ames, Iowa 50011-3111, United States.
J Phys Chem B. 2014 Jul 24;118(29):8471-7. doi: 10.1021/jp412045m. Epub 2014 Jul 3.
Investigation of fluorescence quenching of probes, such as ATTO dyes, is becoming an increasingly important topic owing to the use of these dyes in super-resolution microscopies and in single-molecule studies. Photoinduced electron transfer is their most important nonradiative pathway. Because of the increasing frequency of the use of ATTO and related dyes to investigate biological systems, studies are presented for inter- and intramolecular quenching of ATTO 590 with tryptophan. In order to examine intramolecular quenching, an ATTO 590-tryptophan conjugate was synthesized. It was determined that tryptophan is efficiently quenching ATTO 590 fluorescence by excited-state charge transfer and two charge transfer complexes are forming. In addition, it was discovered that an exciplex (whose lifetime is 5.6 ns) can be formed between tryptophan and ATTO 590, and it is suggested that the possibility of such exciplex formation should be taken into account when protein fluorescence is monitored in a system tagged with ATTO dyes.
由于诸如ATTO染料等探针在超分辨率显微镜和单分子研究中的应用，对其荧光猝灭的研究正成为一个日益重要的课题。光致电子转移是它们最重要的非辐射途径。鉴于使用ATTO及相关染料研究生物系统的频率不断增加，本文展示了关于ATTO 590与色氨酸分子间和分子内猝灭的研究。为了研究分子内猝灭，合成了一种ATTO 590 -色氨酸共轭物。研究确定色氨酸通过激发态电荷转移有效地猝灭了ATTO 590的荧光，并且正在形成两种电荷转移复合物。此外，还发现色氨酸与ATTO 590之间可以形成一种激基复合物（其寿命为5.6纳秒），并表明在用ATTO染料标记的系统中监测蛋白质荧光时，应考虑这种激基复合物形成的可能性。
