口腔鳞状细胞癌中的葡萄糖和谷氨酰胺代谢：来自定量代谢组学方法的见解
Glucose and glutamine metabolism in oral squamous cell carcinoma: insight from a quantitative metabolomic approach.
作者信息
Ogawa Tamaki, Washio Jumpei, Takahashi Tetsu, Echigo Seishi, Takahashi Nobuhiro
机构信息
Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan; Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Aug;118(2):218-25. doi: 10.1016/j.oooo.2014.04.003. Epub 2014 Apr 19.
OBJECTIVE
To characterize the metabolic system of oral squamous cell carcinoma (OSCC) by metabolome analysis.
STUDY DESIGN
The metabolome profiles, including the Embden-Meyerhof-Parnas pathway (EMPP), the pentose phosphate pathway, the tricarboxylic acid cycle (TCAC), and amino acids, were obtained from OSCC and its surrounding normal tissues (32 patients) using capillary electrophoresis and a time-of-flight mass spectrometer.
RESULTS
Enhancement of glucose consumption and lactate production (Warburg effect) was observed in OSCC tissues. The decrease of glucose along with the decrease of the downstream intermediates in the EMPP suggests that incorporated glucose is mainly consumed for biosynthesis. Glutamine consumption with the increase of the intermediates in the last half of the TCAC suggests the involvement of glutaminolysis, in which glutamine is converted to lactate via the last half of the TCAC.
CONCLUSIONS
It is suggested that OSCC tissues show the Warburg effect, which stems from the combined enhancement of glucose consumption and glutaminolysis.
目的
通过代谢组分析来描述口腔鳞状细胞癌（OSCC）的代谢系统。
研究设计
使用毛细管电泳和飞行时间质谱仪，从OSCC及其周围正常组织（32例患者）中获取代谢组谱，包括糖酵解途径（EMPP）、磷酸戊糖途径、三羧酸循环（TCAC）和氨基酸。
结果
在OSCC组织中观察到葡萄糖消耗和乳酸生成增强（瓦伯格效应）。EMPP中葡萄糖及其下游中间体的减少表明，摄入的葡萄糖主要用于生物合成。随着TCAC后半段中间体的增加，谷氨酰胺消耗增加，这表明存在谷氨酰胺分解代谢，其中谷氨酰胺通过TCAC后半段转化为乳酸。
结论
提示OSCC组织表现出瓦伯格效应，这源于葡萄糖消耗和谷氨酰胺分解代谢的共同增强。
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