Webster M D, Weathers W W
Department of Avian Sciences, University of California, Davis 95616.
Am J Physiol. 1989 Feb;256(2 Pt 2):R572-6. doi: 10.1152/ajpregu.1989.256.2.R572.
We have experimentally validated a single-sample variant of the doubly labeled water method for measuring metabolic rate and water turnover in a very small passerine bird, the verdin (Auriparus flaviceps). We measured CO2 production using the Haldane gravimetric technique and compared these values with estimates derived from isotopic data. Doubly labeled water results based on the one-sample calculations differed from Haldane values by less than 0.5% on average (range -8.3 to 11.2%, n = 9). Water flux computed by the single-sample method differed by -1.5% on average from results for the same birds based on the standard, two-sample technique (range -13.7 to 2.0%, n = 9).
我们通过实验验证了双标记水法的单样本变体，用于测量一种非常小的雀形目鸟类——黄头金雀（Auriparus flaviceps）的代谢率和水周转率。我们使用霍尔丹重量法测量了二氧化碳的产生量，并将这些值与从同位素数据得出的估计值进行了比较。基于单样本计算的双标记水结果与霍尔丹值的平均差异小于0.5%（范围为-8.3%至11.2%，n = 9）。通过单样本方法计算的水通量与基于标准双样本技术对同一只鸟的测量结果相比，平均差异为-1.5%（范围为-13.7%至2.0%，n = 9）。
