Komata Makiko, Katou Yuki, Tanaka Hiroshi, Nakato Ryuichiro, Shirahige Katsuhiko, Bando Masashige
Laboratory of Genome Structure and Function, Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan.
Methods Mol Biol. 2014;1164:33-8. doi: 10.1007/978-1-4939-0805-9_4.
The genomic approach (ChIP-seq) we introduce here is now a widely used powerful tool to explore protein-DNA interaction at genome-wide level in high resolution. This technology opens up the way to understand how local event mediated by protein-protein or protein-DNA interactions lead to the dynamic changes of overall chromosome structure and how variety of proteins make a regulatory network for the faithful execution of various chromosomal functions (i.e., transcription, replication, recombination, repair, and partition).
我们在此介绍的基因组方法（ChIP-seq）如今已成为一种广泛应用的强大工具，可在全基因组水平上高分辨率地探索蛋白质与DNA的相互作用。这项技术为理解由蛋白质-蛋白质或蛋白质-DNA相互作用介导的局部事件如何导致整个染色体结构的动态变化，以及各种蛋白质如何构建一个调控网络以确保各种染色体功能（即转录、复制、重组、修复和分配）的忠实执行开辟了道路。
