Egashira T, Obata T, Nagai T, Kimba Y, Takano R, Yamanaka Y
Department of Pharmacology, Medical College of Oita, Japan.
Biochem Pharmacol. 1989 Feb 15;38(4):597-602. doi: 10.1016/0006-2952(89)90204-9.
The extraction and partial purification of endogenous "monoamine oxidase (MAO) inhibitor-like" material from the monkey brain are described. The endogenous material (F-1 and F-2) obtained after Bio-Gel P-2 gel filtration and silica column chromatography inhibited MAO in the monkey brain mitochondria toward 5-hydroxytryptamine (5-HT), beta-phenylethylamine (beta-PEA), tyramine and dopamine as substrates. The inhibitory effects of F-1 and F-2 were non-linear concentration dependent, and F-1 non-competitively inhibited A-form MAO, while F-2 inhibited A-form MAO competitively and inhibited B-form MAO non-competitively. These substances were more potent inhibitors of A-form than of B-form MAO. F-2 was heat stable but liable to the treatment with pepsin and trypsin. F-1 was not inactivated by heat treatment and digestion with pepsin and trypsin. F-1 may be a low molecular weight (less than 1350) compound, including certain monoamines or their metabolites or other unidentified compounds, while F-2 was a low molecular weight (about 2500) peptide.
本文描述了从猴脑中提取和部分纯化内源性“单胺氧化酶（MAO）抑制剂样”物质的过程。经生物凝胶P-2凝胶过滤和硅胶柱色谱法获得的内源性物质（F-1和F-2），能抑制猴脑线粒体中以5-羟色胺（5-HT）、β-苯乙胺（β-PEA）、酪胺和多巴胺为底物的MAO。F-1和F-2的抑制作用呈非线性浓度依赖性，F-1非竞争性抑制A型MAO，而F-2竞争性抑制A型MAO且非竞争性抑制B型MAO。这些物质对A型MAO的抑制作用比对B型MAO更强。F-2热稳定，但易被胃蛋白酶和胰蛋白酶处理。F-1经热处理以及胃蛋白酶和胰蛋白酶消化后不会失活。F-1可能是一种低分子量（小于1350）的化合物，包括某些单胺或其代谢产物或其他未鉴定的化合物，而F-2是一种低分子量（约2500）的肽。
