一种用于定量检测马血浆中降钙素原的新型酶联免疫吸附测定法，其作为马潜在炎症生物标志物。

A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses.

作者信息

Rieger Martin, Kochleus Christian, Teschner Dana, Rascher Daniela, Barton Ann Kristin, Geerlof Arie, Kremmer Elisabeth, Schmid Michael, Hartmann Anton, Gehlen Heidrun

机构信息

Research Unit Microbe-Plant Interactions, Department of Environmental Sciences, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany,

出版信息

Anal Bioanal Chem. 2014 Sep;406(22):5507-12. doi: 10.1007/s00216-014-7944-z. Epub 2014 Jun 14.

DOI:10.1007/s00216-014-7944-z

PMID:24928115

Abstract

In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL(-1) was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL(-1)).

摘要

在人类医学中，降钙素原（PCT）是脓毒症一种非常常见且成熟的生物标志物。尽管脓毒症也是马驹和成年马死亡的主要原因，但截至目前，尚无关于马PCT在患脓毒症马匹中作用的数据。基于针对人PCT的单克隆抗体，我们在此报告一种夹心酶联免疫吸附测定法（ELISA）的开发，用于定量马血浆样本中的马PCT。对该ELISA进行了批内和批间差异分析，定义工作范围为25至1,000 ng mL⁻¹，在此范围内批内和批间差异均低于15%。目标回收率在73%至106%之间。该ELISA用于测定24匹健康马和5匹患脓毒症马的马PCT浓度，以显示马PCT临床评估的潜力。健康对照组和脓毒症组的马PCT平均浓度差异显著（P = 0.0006）（分别为47和8,450 ng mL⁻¹）。