The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3016-3027. doi: 10.1099/ijs.0.062281-0. Epub 2014 Jun 13.
The morphology and infraciliature of two hypotrichous ciliates, Oxytricha paragranulifera n. sp. and Oxytricha granulifera Foissner and Adam, 1983, collected respectively from the surface of a sandy soil in the Huguang mangrove forest, Zhanjiang, China, and the surface of soil in a forest beside Ziwu Road, Xian, north-west China, were examined. O. paragranulifera n. sp. is characterized by an elongate body with slightly tapered anterior end, two macronuclear nodules and two micronuclei, paroral and endoral in Stylonychia-pattern, colourless cortical granules distributed in clusters or irregular short rows, adoral zone occupying 37 % of the body length, marginal rows almost confluent posteriorly, six dorsal kineties and three caudal cirri, caudal cirri and dorsal bristles almost indistinguishable when viewed in vivo. The well-known O. granulifera Foissner and Adam, 1983 was also redescribed and can be separated from the novel species by having cortical granules arranged along dorsal kineties and marginal rows on both sides (vs grouped in clusters as well as in short irregular rows), paroral and endoral in Oxytricha-pattern (vs in Stylonychia-pattern), macronuclear nodules obviously detached (vs adjacent) and a non-saline terrestrial habitat (vs saline terrestrial). The separation of these two taxa is also firmly supported by the molecular data, which show a significant difference between the two in their SSU rRNA gene sequences (similarity 97.1 %). Phylogenetic analyses based on SSU rRNA gene sequence data suggest a close relationship within the Oxytrichidae assemblage between O. paragranulifera n. sp. and O. granulifera.
从中国湛江湖光岩红树林沙土表面和中国西安子午路旁林地土壤表面分别采集的两种旋口目纤毛虫，Oxytricha paragranulifera n. sp. 和 Oxytricha granulifera Foissner 和 Adam，1983，对其形态和纤毛结构进行了研究。O. paragranulifera n. sp. 的特征是身体细长，前端略微变细，有两个大核结节和两个微核，口围带和缘围带呈 Stylonychia 型，无色皮层颗粒呈簇状或不规则短排分布，口前区占体长的 37%，边缘带在后部几乎融合，有 6 条背纤毛带和 3 条尾触毛，活体观察时尾触毛和背刚毛几乎无法区分。著名的 O. granulifera Foissner 和 Adam，1983 也被重新描述，可以通过皮层颗粒沿背纤毛带和两侧边缘带排列（而不是聚集在簇状或不规则的短排中）、口围带和缘围带呈 Oxytricha 型（而不是 Stylonychia 型）、大核结节明显分离（而不是相邻）以及非盐生陆生栖息地（而不是盐生陆生栖息地）与新种区分开来。这些两个分类群的分离也得到了分子数据的有力支持，它们在 SSU rRNA 基因序列上显示出明显的差异（相似性 97.1%）。基于 SSU rRNA 基因序列数据的系统发育分析表明，O. paragranulifera n. sp. 和 O. granulifera 之间在 Oxytrichidae 组合中具有密切的亲缘关系。
