Institut de Parasitologie et de Pathologie Tropicale, EA 7292, Fédération de Médecine Translationelle, Université de Strasbourg, France; Laboratoire de Parasitologie - Mycologie Médicale et Moléculaire (code LR12ES08), Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Tunisia.
Institut de Parasitologie et de Pathologie Tropicale, EA 7292, Fédération de Médecine Translationelle, Université de Strasbourg, France.
Exp Parasitol. 2014 Sep;144:22-6. doi: 10.1016/j.exppara.2014.06.006. Epub 2014 Jun 12.
Müller glial cells are critically involved in retinal inflammatory processes. Here, we investigate the activation of Müller cells in a model of congenital ocular toxoplasmosis (OT). Four weeks after infection, retinal sections were studied immunohistochemically using the markers glial fibrillary acidic protein (GFAP) and vimentin. Müller cells showed strong up-regulation of both markers, as well as a deteriorated morphology in all infected retinas. Moreover, cell density and color intensity of the outer nuclear layer (ONL) of photoreceptors were decreased. Our results indicate that the severe retinal damage and loss of vision observed in human OT may be not only directly caused by infection but rather mediated by infection induced reactive gliosis.
Müller 胶质细胞在视网膜炎症过程中起着至关重要的作用。在这里，我们研究了先天性眼弓形体病 (OT) 模型中 Müller 细胞的激活情况。感染后 4 周，使用胶质纤维酸性蛋白 (GFAP) 和波形蛋白这两种标志物对视网膜切片进行免疫组织化学研究。结果显示，所有感染的视网膜中 Müller 细胞的这两种标志物都呈强烈上调状态，形态也恶化。此外，光感受器的外核层 (ONL) 的细胞密度和颜色强度都降低了。我们的研究结果表明，在人类 OT 中观察到的严重视网膜损伤和视力丧失，不仅可能是由感染直接引起的，而且可能是由感染引起的反应性胶质增生介导的。
