Bioscience Research Institute, Athlone Institute of Technology, Dublin Rd, Athlone, Westmeath, Ireland.
Bioscience Research Institute, Athlone Institute of Technology, Dublin Rd, Athlone, Westmeath, Ireland.
Exp Parasitol. 2014 Sep;144:6-13. doi: 10.1016/j.exppara.2014.06.001. Epub 2014 Jun 11.
Giardia lamblia is a flagellated protozoan parasite that is recognised as a frequent cause of water-borne disease in humans and animals. We report for the first time on the use of a combined in vitro HCT-8 cell culture-quantitative PCR assay for evaluating the efficacy of using pulsed UV light for treating G. lamblia parasites. Findings showed that current methods that are limited to using vital stains before and after cyst excystation are not appropriate for monitoring or evaluating cyst destruction post PUV-treatments. Use of the human ileocecal HCT-8 cell line was superior to that of the human colon Caco-2 cell line for in vitro culture and determining PUV sensitivity of treated cysts. G. lamblia cysts were also shown to be more resistant to PUV irradiation compared to treating similar numbers of Cryptosporidium parvum oocysts. These observations also show that the use of this HCT-8 cell culture assay may replace use of animal models for determining disinfection performances of PUV for treating both C. parvum and G. lamblia.
蓝氏贾第鞭毛虫是一种有鞭毛的原生动物寄生虫，被认为是人类和动物中常见的水源性疾病的原因。我们首次报告了一种联合使用体外 HCT-8 细胞培养定量 PCR 检测方法，以评估脉冲紫外光处理蓝氏贾第鞭毛虫寄生虫的效果。研究结果表明，目前仅限于在囊孢囊形成前后使用活体染色的方法并不适合监测或评估 PUV 处理后囊泡的破坏。与人类结肠 Caco-2 细胞系相比，人回肠 HCT-8 细胞系更适合用于体外培养和确定处理后的囊泡对 PUV 的敏感性。与处理数量相似的隐孢子虫卵囊相比，蓝氏贾第鞭毛虫囊泡也对 PUV 照射具有更强的抵抗力。这些观察结果还表明，该 HCT-8 细胞培养检测方法的使用可能取代动物模型，用于确定 PUV 对治疗隐孢子虫和蓝氏贾第鞭毛虫的消毒性能。
