Department of Aquatic Life and Medical Sciences, Sun Moon University, Asan-si, Chungnam 336-708, Republic of Korea.
Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea.
Exp Parasitol. 2014 Sep;144:34-8. doi: 10.1016/j.exppara.2014.06.007. Epub 2014 Jun 12.
Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by Miamiensis avidus in olive flounder (Paralichthys olivaceus) was investigated. The auxotrophic E. tarda mutant with pL-CpG30 plasmids elicited evidently higher survival rates and delayed both onset of mortality and time-to-death of olive flounder against M. avidus challenge. Furthermore, administration of E. tarda mutant that contains plasmids enriched in CpG 1668 motif elicited clearly higher survival rates of fish that were already infected with M. avidus. These results indicate that CpG 1668 plasmids-harboring E. tarda mutant may provide not only preventive measure but also therapeutic approach against scuticociliatosis in olive flounder.
先前生成的两种营养缺陷型基因敲除迟钝爱德华氏菌（Δalr Δasd E. tarda）被用作富含 CpG 1668 基序的质粒（pL-CpG30）的递送载体，并且研究了Δalr Δasd E. tarda 介导的 CpG 基序在预防和治疗由 Miamiensis avidus 引起的橄榄石斑鱼（Paralichthys olivaceus）的盾纤毛虫病中的潜力。携带 pL-CpG30 质粒的营养缺陷型 E. tarda 突变体引起的橄榄石斑鱼的存活率明显更高，并且延迟了死亡率的开始和死亡时间对 M. avidus 挑战的影响。此外，施用富含 CpG 1668 基序的质粒的 E. tarda 突变体可明显提高已感染 M. avidus 的鱼类的存活率。这些结果表明，含有 CpG 1668 质粒的 E. tarda 突变体不仅可以提供预防措施，而且可以为橄榄石斑鱼的盾纤毛虫病提供治疗方法。
