Sprangers S A, Brenner R M, Bethea C L
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
Endocrinology. 1989 Mar;124(3):1462-70. doi: 10.1210/endo-124-3-1462.
The distribution of estrogen receptors (ER), progesterone receptors (PR), PRL, and gonadotropins in different cell types of the monkey pituitary was examined by immunocytochemical (ICC) labeling of pituitary cell cultures. Dispersed monkey pituitary cells were cultured on extracellular matrix and in serum-free medium for 6-14 days. Individual cultures were singly stained for ER, PR, PRL, LH, and FSH or double labeled for PR and one of the protein hormones. ICC reaction product localizes over the nuclei of cells that are positive for the steroid receptors, whereas reaction product localizes over the cytoplasm of cells that are positive for the protein hormones. Sixty-one percent of the parenchymal cells were positive for PRL, while 1-3% were positive for LH or FSH. Sixty-two percent of the parenchymal cells were ER positive. ER staining was localized over the nuclei of two morphologically distinct cell types. One cell type is smaller and more prevalent than the second cell type. Based on single staining for each of the protein hormones, we propose that the smaller cells are lactotropes, and the larger cells are gonadotropes. PR-positive cells averaged 7.7% of the parenchymal cells. Double ICC staining for PR and the protein hormones demonstrated that PR localize in the nuclei of gonadotropes, but not lactotropes, of monkey pituitary cell cultures. The absence of PR in lactotropes is consistent with our observation that progesterone has no direct effect on PRL secretion in monkey pituitary cell cultures. In contrast, the presence of PR in gonadotropes suggests that progesterone may act directly at the pituitary to modulate gonadotropin secretion in the primate. In conclusion, ER are present in both lactotropes and gonadotropes. PR are present in gonadotropes, but not lactotropes, of the primate pituitary.
通过对垂体细胞培养物进行免疫细胞化学（ICC）标记，研究了雌激素受体（ER）、孕激素受体（PR）、催乳素（PRL）和促性腺激素在猴垂体不同细胞类型中的分布。将分散的猴垂体细胞在细胞外基质上和无血清培养基中培养6 - 14天。对单个培养物分别进行ER、PR、PRL、促黄体生成素（LH）和促卵泡生成素（FSH）染色，或对PR与一种蛋白质激素进行双重标记。ICC反应产物定位于对类固醇受体呈阳性的细胞的细胞核上，而反应产物定位于对蛋白质激素呈阳性的细胞的细胞质上。61%的实质细胞PRL呈阳性，而1 - 3%的细胞LH或FSH呈阳性。62%的实质细胞ER呈阳性。ER染色定位于两种形态不同的细胞类型的细胞核上。一种细胞类型比第二种细胞类型小且更普遍。基于对每种蛋白质激素的单染色，我们认为较小的细胞是催乳细胞，较大的细胞是促性腺细胞。PR阳性细胞平均占实质细胞的7.7%。对PR和蛋白质激素进行双重ICC染色表明，在猴垂体细胞培养物中，PR定位于促性腺细胞的细胞核，而不是催乳细胞的细胞核。催乳细胞中不存在PR与我们观察到的孕激素对猴垂体细胞培养物中PRL分泌无直接影响一致。相反，促性腺细胞中存在PR表明孕激素可能直接作用于垂体以调节灵长类动物的促性腺激素分泌。总之，ER存在于催乳细胞和促性腺细胞中。PR存在于灵长类动物垂体的促性腺细胞中，而不存在于催乳细胞中。
