Zhang Li, Zhang Xianhong
School of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, PR China.
College of Agriculture, Shanxi Agricultural University, Taigu 030801, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:54-9. doi: 10.1016/j.saa.2014.04.130. Epub 2014 Apr 30.
A novel fluorescein derivative 3-bromo-5-methylsalicylaldehyde fluorescein hydrazone (BMSFH) has been synthesized by reacting fluorescein hydrazide with 3-bromo-5-methylsalicylaldehyde and was developed as a new colorimetric probe for detection of Cu(2+). In the presence of Cu(2+) the BMSFH exhibits a rapid color change from colorless to yellow together with an obvious new band appeared at 502nm in the UV-vis absorption spectra. However, other common alkali-, alkaline earth-, transition- and rare earth metal ions induced no or minimal spectral changes. This change is attributed to BMSFH via coordination with Cu(2+) in a 1:1 stoichiometry and this binding to Cu(2+) is reversible, as indicated by the bleaching of the color when the Cu(2+) is extracted with EDTA. Experimental results indicate that the BMSFH can provide a rapid, selective and sensitive response to Cu(2+) with a linear dynamic range 3.0-330μmol/L and can be used as a potential Cu(2+) colorimetric probe in aqueous solution.
通过使荧光素酰肼与3-溴-5-甲基水杨醛反应合成了一种新型荧光素衍生物3-溴-5-甲基水杨醛荧光素腙（BMSFH），并将其开发为一种用于检测Cu(2+)的新型比色探针。在Cu(2+)存在下，BMSFH呈现出从无色到黄色的快速颜色变化，并且在紫外可见吸收光谱中502nm处出现明显的新谱带。然而，其他常见的碱金属、碱土金属、过渡金属和稀土金属离子未引起或仅引起最小的光谱变化。这种变化归因于BMSFH与Cu(2+)以1:1化学计量比配位，并且这种与Cu(2+)的结合是可逆的，当用EDTA萃取Cu(2+)时颜色会褪去表明了这一点。实验结果表明，BMSFH对Cu(2+)能提供快速、选择性和灵敏的响应，线性动态范围为3.0 - 330μmol/L，可作为水溶液中潜在的Cu(2+)比色探针。
