Khodadoust Saeid, Ghaedi Mehrorang
Department of Chemistry, Yasouj University, Yasouj 75914-35, Iran.
Department of Chemistry, Yasouj University, Yasouj 75914-35, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:87-92. doi: 10.1016/j.saa.2014.04.119. Epub 2014 Apr 30.
In this study a rapid and effective method (dispersive liquid-liquid microextraction (DLLME)) was developed for extraction of methyl red (MR) prior to its determination by UV-Vis spectrophotometry. Influence variables on DLLME such as volume of chloroform (as extractant solvent) and methanol (as dispersive solvent), pH and ionic strength and extraction time were investigated. Then significant variables were optimized by using a Box-Behnken design (BBD) and desirability function (DF). The optimized conditions (100μL of chloroform, 1.3mL of ethanol, pH 4 and 4% (w/v) NaCl) resulted in a linear calibration graph in the range of 0.015-10.0mgmL(-1) of MR in initial solution with R(2)=0.995 (n=5). The limits of detection (LOD) and limit of quantification (LOQ) were 0.005 and 0.015mgmL(-1), respectively. Finally, the DLLME method was applied for determination of MR in different water samples with relative standard deviation (RSD) less than 5% (n=5).
在本研究中，开发了一种快速有效的方法（分散液液微萃取（DLLME）），用于在通过紫外可见分光光度法测定甲基红（MR）之前对其进行萃取。研究了诸如氯仿（作为萃取溶剂）和甲醇（作为分散溶剂）的体积、pH值、离子强度以及萃取时间等对DLLME的影响变量。然后，通过使用Box-Behnken设计（BBD）和期望函数（DF）对显著变量进行了优化。优化条件（100μL氯仿、1.3mL乙醇、pH 4和4%（w/v）NaCl）在初始溶液中MR浓度为0.015 - 10.0mgmL⁻¹范围内产生了线性校准曲线，R² = 0.995（n = 5）。检测限（LOD）和定量限（LOQ）分别为0.005和0.015mgmL⁻¹。最后，将DLLME方法应用于不同水样中MR的测定，相对标准偏差（RSD）小于5%（n = 5）。
