Liu Hai-gang, Li Hong-mei, Wang Shu-ying, Huang Li-bo, Guo Hui-jun
Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Comput Biol Chem. 2014 Aug;51:42-50. doi: 10.1016/j.compbiolchem.2014.04.002. Epub 2014 Jun 2.
To clarify the molecular evolution and characteristic of beta estrogen receptor (ERβ) gene in Jining Gray goat in China, the entire ERβ gene from Jining Gray goat ovary was amplified, identified and sequenced, and the gene sequences were compared with those of other animals. Functional structural domains and variations in DNA binding domains (DBD) and ligand binding domains (LBD) between Jining Gray goat and Boer goat were analyzed. The results indicate that the ERβ gene in Jining Gray goat includes a 1584bp sequence with a complete open-reading-frame (ORF), encoding a 527 amino acid (aa) receptor protein. Compared to other species, the nucleotide homology is 73.9-98.9% and the amino acid homology is 79.5-98.5%. The main antigenic structural domains lie from the 97th aa to the 286th aa and from the 403rd aa to the 527th aa. The hydrophilicity and the surface probability of the structural domains are distributed throughout a range of amino acids. There are two different amino acids in the DBD and three different amino acids in the LBD between Jining Gray and Boer goats, resulting in dramatically different spatial structures for ERβ protein. These differences may explain the different biological activities of ERβ between the two goat species. This study firstly acquired the whole ERβ gene sequence of Jining Gray goat with a complete open reading frame, and analyzed its gene evolutionary relationship and predicted its mainly functional structural domains, which may very help for further understanding the genome evolution and gene diversity of goat ERβ.
为阐明中国济宁青山羊β雌激素受体（ERβ）基因的分子进化及特征，扩增、鉴定并测序了济宁青山羊卵巢中的完整ERβ基因，并将该基因序列与其他动物的进行比较。分析了济宁青山羊和波尔山羊之间DNA结合域（DBD）和配体结合域（LBD）的功能结构域及变异情况。结果表明，济宁青山羊的ERβ基因包含一个1584bp的序列，具有完整的开放阅读框（ORF），编码一个527个氨基酸（aa）的受体蛋白。与其他物种相比，核苷酸同源性为73.9 - 98.9%，氨基酸同源性为79.5 - 98.5%。主要抗原结构域位于第97个氨基酸至第286个氨基酸以及第403个氨基酸至第527个氨基酸处。结构域的亲水性和表面概率分布在一系列氨基酸范围内。济宁青山羊和波尔山羊的DBD中有两个不同的氨基酸，LBD中有三个不同的氨基酸，导致ERβ蛋白的空间结构显著不同。这些差异可能解释了两种山羊之间ERβ的不同生物学活性。本研究首次获得了具有完整开放阅读框的济宁青山羊全ERβ基因序列，分析了其基因进化关系并预测了其主要功能结构域，这可能对进一步了解山羊ERβ的基因组进化和基因多样性有很大帮助。
