Gao Su, Wang Wei, Tian Hua, Zhang Xiaona, Guo Linlin, Ru Shaoguo
Marine Life Science College, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Shandong, People's Republic of China.
Bull Environ Contam Toxicol. 2014 Sep;93(3):280-8. doi: 10.1007/s00128-014-1305-7. Epub 2014 Jun 15.
To determine the endocrine disrupting effect of semicarbazide, an emerging water contaminant, the changes in transcript levels of hepatic estrogen-response genes including vitellogenin-1 (vtg-1), estrogen receptor α (ERα), and estrogen receptor β (ERβ) were measured in male and female zebrafish exposed to semicarbazide with or without exogenous 17β-estradiol (E2). Exposure of male zebrafish to semicarbazide for 96 h or 28 days resulted in no significant induction in hepatic vtg-1, ERα, or ERβ mRNA expression, indicating that semicarbazide has no estrogenic effect. However, a remarkable anti-estrogenic effect of semicarbazide was demonstrated: semicarbazide treatment of female zebrafish for 96 h and 28 days resulted in significant decreases in transcript levels of vtg-1, ERα, and ERβ, as well as decreases in the gonadosomatic index level after 28 days. Moreover, semicarbazide exposure significantly inhibited the induction of vtg-1, ERα and ERβ mRNA by E2 when male zebrafish were co-exposed for 28 days.
为了确定新型水体污染物氨基脲的内分泌干扰效应，我们检测了暴露于氨基脲（添加或不添加外源性17β-雌二醇（E2））的雄性和雌性斑马鱼肝脏雌激素反应基因转录水平的变化，这些基因包括卵黄蛋白原-1（vtg-1）、雌激素受体α（ERα）和雌激素受体β（ERβ）。雄性斑马鱼暴露于氨基脲96小时或28天，肝脏vtg-1、ERα或ERβ mRNA表达均未出现显著诱导，表明氨基脲无雌激素效应。然而，氨基脲显示出显著的抗雌激素作用：雌性斑马鱼经氨基脲处理96小时和28天，vtg-1、ERα和ERβ转录水平显著降低，28天后性腺指数水平也降低。此外，当雄性斑马鱼共同暴露28天时，氨基脲暴露显著抑制了E2对vtg-1、ERα和ERβ mRNA的诱导。
