Muller E G, Buchanan B B
Division of Molecular Plant Biology, University of California, Berkeley 94720.
J Biol Chem. 1989 Mar 5;264(7):4008-14.
We have taken advantage of the transformation properties of the cyanobacterium Anacystis nidulans R2 to investigate the importance of thioredoxin for photosynthetic growth. The gene encoding thioredoxin m, designated trxM, was cloned from A. nidulans using a synthetic oligonucleotide probe. Based on the nucleotide sequence, thioredoxin m of A. nidulans is composed of 107 amino acids and shares 84, 48, and 48% sequence identity with thioredoxins from Anabaena, spinach, and Escherichia coli, respectively. The trxM gene is single copy and is transcribed on a 510-nucleotide mRNA. We demonstrate that disruption of the trxM gene with a kanamycin resistance gene cartridge is a lethal mutation. Although dispensable in E. coli, thioredoxin is essential for the photosynthetic growth of A. nidulans.
我们利用蓝藻集胞藻6803 R2的转化特性来研究硫氧还蛋白对光合生长的重要性。使用合成寡核苷酸探针从集胞藻中克隆了编码硫氧还蛋白m的基因，命名为trxM。根据核苷酸序列，集胞藻的硫氧还蛋白m由107个氨基酸组成，分别与鱼腥藻、菠菜和大肠杆菌的硫氧还蛋白具有84%、48%和48%的序列同一性。trxM基因是单拷贝的，转录在一个510个核苷酸的mRNA上。我们证明用卡那霉素抗性基因盒破坏trxM基因是一个致死突变。虽然硫氧还蛋白在大肠杆菌中是可有可无的，但它对集胞藻的光合生长至关重要。
