对无特定病原体鸡蛋进行高通量测序分析。

Analysis by high throughput sequencing of Specific Pathogen Free eggs.

作者信息

Gagnieur Léa, Cheval Justine, Cochet Marielle, Breard Emmanuel, Gratigny Marlène, Hébert Charles, Muth Erika, Viarouge Cyril, Dumarest Marine, Coulpier Muriel, Eloit Marc

机构信息

Institut Pasteur, Laboratory of Pathogen Discovery, Department of Virology, 28 rue du Docteur Roux, F-75724 Paris, France.

PathoQuest, Bâtiment François Jacob, 25 rue du Dr Roux, 75015, Paris, France.

出版信息

Biologicals. 2014 Jul;42(4):218-9. doi: 10.1016/j.biologicals.2014.05.004. Epub 2014 Jun 13.

DOI:10.1016/j.biologicals.2014.05.004

PMID:24930452

Abstract

Specific Pathogen Free (SPF) embryonated eggs are used for the production of many veterinary and human vaccines. We have used High Throughput Sequencing to screen allantoic fluids and embryos for the presence of encapsidated viral genomes and viral transcripts, respectively. SPF eggs from two different producers were tested. We evidenced sequences corresponding to known endogenous retroviruses and sequences of Avian Leukosis Virus, but no sequence that might suggest a productive infection of eggs with a virus even distant from known viruses. Our results strongly suggest that SPF eggs such as those used for this study represent a safe substrate for the production of vaccines.

摘要

无特定病原体（SPF）的受精蛋被用于生产多种兽用和人用疫苗。我们利用高通量测序技术分别筛查尿囊液和胚胎中是否存在衣壳化病毒基因组和病毒转录本。对来自两个不同供应商的SPF蛋进行了检测。我们发现了与已知内源性逆转录病毒相对应的序列以及禽白血病病毒的序列，但没有序列表明蛋被哪怕与已知病毒亲缘关系很远的病毒进行了有效感染。我们的结果有力地表明，像本研究中所使用的这类SPF蛋是生产疫苗的安全基质。