Cockle S M, Morrell J M, Smyth D G
National Institute for Medical Research, Mill Hill, London.
J Endocrinol. 1989 Jan;120(1):31-6. doi: 10.1677/joe.0.1200031.
TRH-related peptides were extracted from the hypothalamus and prostate gland of the rabbit. The peptides were fractionated by gel exclusion chromatography and located by trypsin digestion and radioimmunoassay with antibodies to TRH amide and TRH-Gly Lys. In the hypothalamus TRH-related peptides containing approximately 16 and 30 residues were observed: in these peptides the extensions to the TRH sequence were exclusively in the C-terminal direction. In addition, the three-residue form of TRH was also present. In the prostate complex, the predominant TRH-related peptide contained approximately 50 residues and the extension to the TRH tripeptide was on the N-terminal side; a three-residue form of immunoreactive TRH was also demonstrated. The same pattern of TRH-related peptides was shown to be present in rabbit semen. The results reveal the existence of a novel TRH-related polypeptide in the prostate and semen which does not occur in the hypothalamus. This peptide appears to undergo secretion.
从兔子的下丘脑和前列腺中提取了促甲状腺激素释放激素（TRH）相关肽。这些肽通过凝胶排阻色谱法进行分级分离，并通过胰蛋白酶消化以及使用针对TRH酰胺和TRH-甘氨酸赖氨酸的抗体进行放射免疫测定来定位。在下丘脑中观察到含有约16和30个残基的TRH相关肽：在这些肽中，TRH序列的延伸仅在C末端方向。此外，还存在TRH的三残基形式。在前列腺复合体中，主要的TRH相关肽含有约50个残基，TRH三肽的延伸在N末端一侧；还证实了免疫反应性TRH的三残基形式。兔子精液中也显示出相同模式的TRH相关肽。结果揭示了在前列腺和精液中存在一种下丘脑不存在的新型TRH相关多肽。这种肽似乎会发生分泌。
