Weis M T, Malik K U
Department of Pharmacology, University of Tennessee, Memphis.
J Pharmacol Exp Ther. 1989 Feb;248(2):614-20.
Tritiated phosphatidyl choline (1-palmitoyl,2-[3H]palmitoyl or 1-stearoyl,2-[3H]arachidonyl) is taken up unchanged by the isolated perfused rabbit heart. Isoproterenol, vasopressin or A23187 enhanced the output of tritium in these hearts, whereas bradykinin, angiotensin II or acetylcholine had no effect. These data demonstrate that [3H]phosphatidyl choline is incorporated directly into a phospholipid pool that is accessible to some, but not all, hormonally stimulated lipases. In hearts labeled with 1-stearoyl,2-[3H]arachidonyl phosphatidyl choline, the radioactivity released by isoproterenol consisted of free fatty acid and lysophosphatidyl choline, indicating that isoproterenol stimulates both phospholipase(s) A1 and A2. Vasopressin and A23187 increased the release of free fatty acid, but not lysophosphatidyl choline, suggesting that these agents stimulate only phospholipase A2.
氚标记的磷脂酰胆碱（1-棕榈酰基，2-[3H]棕榈酰基或1-硬脂酰基，2-[3H]花生四烯酰基）被离体灌注的兔心脏原样摄取。异丙肾上腺素、血管加压素或A23187可增加这些心脏中氚的释放量，而缓激肽、血管紧张素II或乙酰胆碱则无此作用。这些数据表明，[3H]磷脂酰胆碱直接掺入到一个磷脂池中，某些但并非所有受激素刺激的脂肪酶都可作用于该磷脂池。在用1-硬脂酰基，2-[3H]花生四烯酰基磷脂酰胆碱标记的心脏中，异丙肾上腺素释放的放射性物质包括游离脂肪酸和溶血磷脂酰胆碱，这表明异丙肾上腺素可刺激磷脂酶A1和A2。血管加压素和A23187增加了游离脂肪酸的释放，但未增加溶血磷脂酰胆碱的释放，这表明这些药物仅刺激磷脂酶A2。
