用小干扰RNA沉默RIP1对人结肠癌LoVo细胞系生物学行为的影响。

Effects of silencing RIP1 with siRNA on the biological behavior of the LoVo human colon cancer cell line.

作者信息

You Hong-Xia, Zhou Yan-Hong, Tan Shi-Yun, She Tong-Hui

机构信息

Department of Gastroenterology, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China ; Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Department of Gastroenterology, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.

出版信息

Oncol Lett. 2014 Jun;7(6):2065-2072. doi: 10.3892/ol.2014.2040. Epub 2014 Apr 7.

DOI:10.3892/ol.2014.2040

PMID:24932290

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4049674/

Abstract

The present study aimed to investigate the effects of silencing RIP1 by small interfering RNA (siRNA) on the biological behavior of the LoVo human colorectal carcinoma cell line and to provide evidence for the feasibility of colorectal cancer gene therapy. LoVo cells were divided into the RIP1 siRNA group, the blank control group and the negative control group. Chemically synthesized siRNA targeting RIP1 (RIP1 siRNA) was transfected into LoVo cells. Following transfection of the RIP1-targeted siRNA into the LoVo cells, the expression of the RIP1 gene was effectively inhibited. The results demonstrated that RIP1 effectively regulated the malignant biological behavior of the LoVo colon cancer cell line. Furthermore, the proliferation, motility and invasiveness of LoVo cells were inhibited by siRNA knockdown of RIP1. The results revealed that the RIP1 gene has an important role in the regulation of proliferation and apoptosis in colorectal carcinoma cells.

摘要

本研究旨在探讨小干扰RNA（siRNA）沉默RIP1对人结肠癌细胞系LoVo生物学行为的影响，并为结直肠癌基因治疗的可行性提供依据。将LoVo细胞分为RIP1 siRNA组、空白对照组和阴性对照组。将化学合成的靶向RIP1的siRNA（RIP1 siRNA）转染到LoVo细胞中。将靶向RIP1的siRNA转染到LoVo细胞后，RIP1基因的表达受到有效抑制。结果表明，RIP1有效地调节了LoVo结肠癌细胞系的恶性生物学行为。此外，RIP1的siRNA敲低抑制了LoVo细胞的增殖、运动性和侵袭性。结果显示，RIP1基因在调节结肠癌细胞的增殖和凋亡中具有重要作用。