细胞诱导的明胶甲基丙烯酸酯微滴生成中的流聚焦不稳定性。
Cell-induced flow-focusing instability in gelatin methacrylate microdroplet generation.
机构信息
Hemorheology Research Institute, Chonbuk National University, Jeonju 561-756, South Korea.
Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, South Korea.
出版信息
Biomicrofluidics. 2014 May 27;8(3):036503. doi: 10.1063/1.4880375. eCollection 2014 May.
Photo-crosslinkable gelatin methacrylate (GelMa) microspheres are applicable to deliver cells or drugs in biological or biomedical applications. To fabricate GelMa microdroplets, a flow focusing technique with advantages of size control and rapid production was used in a T-junction microfluidic device. Instability played an important role in promoting microdroplet uniformity. 5 wt. % GelMa prepolymer solution mixed with cells affected cell-induced instability. At low flow rate ratio of GelMa to mineral oil below 0.200, stability was maintained regardless of GelMa concentration (5 and 8 wt. %) and cell presence, which led to uniform microdroplet generation. In contrast, instability at high flow rate ratio above 0.200 was worsened by cell presence and unstable jetting length, resulting in the generation of non-uniform cell-laden microdroplets. Therefore, the effect of cell-induced instability on microdroplet generation was minimized at a low flow rate ratio.
光交联明胶甲基丙烯酸酯（GelMa）微球适用于在生物或生物医学应用中输送细胞或药物。为了制备 GelMa 微液滴，在 T 形 Junction 微流控装置中使用具有尺寸控制和快速生产优点的流聚焦技术。不稳定性在促进微液滴均匀性方面起着重要作用。将细胞与 5wt.% GelMa 预聚物溶液混合会影响细胞诱导的不稳定性。在 GelMa 与矿物油的流速比低于 0.200 的低流速比下，无论 GelMa 浓度（5wt.%和 8wt.%）和细胞存在与否，都能保持稳定性，从而产生均匀的微液滴。相比之下，在高流速比（高于 0.200）下，细胞存在和不稳定的射流长度会加剧不稳定性，导致非均匀的载细胞微液滴的生成。因此，在低流速比下，细胞诱导的不稳定性对微液滴生成的影响最小化。
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