分析总空气细菌浓度的变化，以评估微生物实验室中生物安全柜的性能。
Analysis of variation in total airborne bacteria concentration to assess the performance of biological safety cabinets in microbial laboratories.
机构信息
Department of Occupational and Environmental Medicine, Ajou University School of Medicine, Suwon, Korea.
Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency, Incheon, Korea.
出版信息
Saf Health Work. 2014 Mar;5(1):23-6. doi: 10.1016/j.shaw.2014.01.001. Epub 2014 Jan 24.
BACKGROUND
The purpose of this study was to compare the concentration of total airborne bacteria (TAB) in biosafety cabinets (BSCs) at universities and hospital microbial laboratories to assess the performance of BSCs.
METHODS
TAB was determined by using the single-stage Anderson sampler (BioStage Viable Cascade Impactor). The samples were obtained three times (with the BSC turned off and the shield open; with the BSC turned off and the shield closed; and with the BSC tuned on and operating) from the areas in front of 11 BSCs.
RESULTS
TAB concentrations of accredited and nonaccredited BSCs were determined. No significant differences were observed in the TAB concentrations of the accredited BSCs and the nonaccredited BSCs for the areas outside the BSCs in the laboratories (p > 0.05). TAB concentrations for the BSCs sampled with the shield open and the instrument turned off showed differences based on the sampling site outside the BSC in each laboratory.
CONCLUSION
These results imply that TAB concentration is not altered by the performance of the BSCs or TAB itself and/or concentration of TAB outside the BSC is not a good index of BSC performance.
背景
本研究旨在比较大学和医院微生物实验室生物安全柜（BSC）中总空气细菌（T AB）的浓度，以评估 BSC 的性能。
方法
采用单级安德森采样器（BioStage 可培养级联撞击器）测定 T AB。从 11 个 BSC 前方区域，BSC 关闭且屏蔽门打开、BSC 关闭且屏蔽门关闭以及 BSC 开启且运行三种状态下，采集三次样本。
结果
确定了经过认证和未经认证的 BSC 的 T AB 浓度。在实验室 BSC 外部区域，经认证和未经认证的 BSC 的 T AB 浓度无显著差异（p>0.05）。对于屏蔽门打开且仪器关闭时采集的 BSC 样本，每个实验室的 BSC 外部采样点的 T AB 浓度存在差异。
结论
这些结果表明，BSC 的性能或 T AB 本身及其浓度不会改变 T AB 浓度，并且/或者 BSC 外部的 T AB 浓度不是 BSC 性能的良好指标。
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