Hämmerle Hermann, Amman Fabian, Večerek Branislav, Stülke Jörg, Hofacker Ivo, Bläsi Udo
Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, Centre of Molecular Biology, University of Vienna, Vienna, Austria.
Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria.
PLoS One. 2014 Jun 16;9(6):e98661. doi: 10.1371/journal.pone.0098661. eCollection 2014.
The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival advantage. A comparative transcriptome analysis revealed mRNAs with a differential abundance that are governed by the ResD-ResE system required for aerobic and anaerobic respiration. Expression of resD was found to be up-regulated in the hfq- strain. Furthermore, several genes of the GerE and ComK regulons were de-regulated in the hfq- background. Surprisingly, only six out of >100 known and predicted small RNAs (sRNAs) showed altered abundance in the absence of Hfq. Moreover, Hfq positively affected the transcript abundance of genes encoding type I toxin-antitoxin systems. Taken the moderate effect on sRNA levels and mRNAs together, it seems rather unlikely that Hfq plays a central role in RNA transactions in Bacillus subtilis.
RNA伴侣蛋白Hfq在多种革兰氏阴性菌的转录后基因调控中发挥核心作用，而关于其在革兰氏阳性菌中的作用却知之甚少。在此，我们研究了枯草芽孢杆菌中Hfq的功能，并表明它赋予了生存优势。一项比较转录组分析揭示了由有氧和无氧呼吸所需的ResD-ResE系统调控的丰度有差异的mRNA。发现resD在hfq-菌株中的表达上调。此外，GerE和ComK调控子的几个基因在hfq-背景下失调。令人惊讶的是，在100多个已知和预测的小RNA（sRNA）中，只有6个在没有Hfq的情况下丰度发生了变化。此外，Hfq对编码I型毒素-抗毒素系统的基因的转录本丰度有正向影响。综合对sRNA水平和mRNA的适度影响来看，Hfq在枯草芽孢杆菌的RNA交易中似乎不太可能发挥核心作用。
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