Peng Anqi, Zhang Weijiao, Xiong Haibo, Zhang Luyao, Cheng Jian, Wang Yang, Kang Zhen
The Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, Wuxi 214122, China.
Eng Microbiol. 2024 Dec 9;5(1):100186. doi: 10.1016/j.engmic.2024.100186. eCollection 2025 Mar.
Small regulatory RNAs (sRNAs) are non-coding RNA molecules that fine-tune various cellular processes and respond to various environmental stimuli. In , the regulatory mechanisms and specific targets of several sRNAs remain largely unknown. In this study, we identified and characterized S612 as a self-terminating sRNA in . The expression of S612 is regulated by external signals, including nutrient availability and salt concentration. Overexpression of S612 induced filamentous cells with extensive cellular elongation and complete inhibition of sporulation, indicating its potential to control cell morphology and spore formation. S612 directly targets and downregulates genes through post-transcriptional base pairing with mRNAs, including , and , some of which are involved in cell membrane integrity, cell wall synthesis, and sporulation initiation. Therefore, we propose that S612 is an important post-transcriptional regulator of cell morphology and sporulation.
小调控RNA（sRNA）是非编码RNA分子，可微调各种细胞过程并响应各种环境刺激。然而，几种sRNA的调控机制和特定靶标在很大程度上仍然未知。在本研究中，我们鉴定并表征了S612为[具体物种或系统]中的一种自终止sRNA。S612的表达受外部信号调控，包括营养可用性和盐浓度。S612的过表达诱导丝状细胞，伴有广泛的细胞伸长和孢子形成的完全抑制，表明其具有控制细胞形态和孢子形成的潜力。S612通过与mRNA进行转录后碱基配对直接靶向并下调基因，包括[具体基因名称1]、[具体基因名称2]和[具体基因名称3]，其中一些基因参与细胞膜完整性、细胞壁合成和孢子形成起始。因此，我们提出S612是细胞形态和孢子形成的重要转录后调节因子。
