Leroy A, Olivier P, Vu-Dac N, Fruchart J C
SERLIA, Institut Pasteur, Lille, France.
Clin Chim Acta. 1989 Jan 13;179(1):85-95. doi: 10.1016/0009-8981(89)90026-0.
Two monoclonal antibodies against human apolipoprotein A-I were characterized to recognize rabbit apo A-I. By immunoblotting we determined that while neither antibody reacted with the other rabbit apolipoproteins they both recognized all rabbit apo A-I isoforms. Cotitration revealed that each antibody bound to different apo A-I epitopes. Then we developed a noncompetitive enzyme-linked immunosorbent assay (ELISA) to measure the concentration of total apolipoprotein A-I in rabbit serum. The ELISA curves of the different lipoprotein class and serum showed the similarity of the expression of the apo A-I epitopes recognized in each of them and in serum. This assay, as opposed to other techniques, offers several advantages such as sensitivity, specificity, and simplicity and avoids the use of radioisotope.
对两种抗人载脂蛋白A-I的单克隆抗体进行了表征，以确定它们对兔载脂蛋白A-I的识别情况。通过免疫印迹，我们确定，虽然这两种抗体均不与其他兔载脂蛋白发生反应，但它们都能识别所有兔载脂蛋白A-I同工型。协同滴定显示，每种抗体与不同的载脂蛋白A-I表位结合。然后，我们开发了一种非竞争性酶联免疫吸附测定法（ELISA）来测量兔血清中总载脂蛋白A-I的浓度。不同脂蛋白类别和血清的ELISA曲线显示了其中每种物质以及血清中所识别的载脂蛋白A-I表位表达的相似性。与其他技术相比，该测定法具有灵敏度、特异性和简便性等诸多优点，并且无需使用放射性同位素。
