Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Biomaterials. 2014 Aug;35(26):7363-73. doi: 10.1016/j.biomaterials.2014.05.019. Epub 2014 Jun 2.
Natural and synthetic three-dimensional (3-D) scaffolds that mimic the microenvironment of the extracellular matrix (ECM), with growth factor storage/release and the display of cell adhesion signals, offer numerous advantages for regenerative medicine and in vitro morphogenesis and oncogenesis modeling. Here we report the design of collagen mimetic peptides (CMPs) that assemble into a highly crosslinked 3-D matrix in response to metal ion stimuli, that may be functionalized with His-tagged cargoes, such as green fluorescent protein (GFP-His8) and human epidermal growth factor (hEGF-His6). The bound hEGF-His6 was found to gradually release from the matrix in vitro and induce cell proliferation in the EGF-dependent cell line MCF10A. The additional incorporation of a cell adhesion sequence (RGDS) at the N-terminus of the CMP creates an environment that facilitated the organization of matrix-encapsulated MCF10A cells into spheroid structures, thus mimicking the ECM environment.
天然和合成的三维（3-D）支架模仿细胞外基质（ECM）的微环境，具有生长因子储存/释放和细胞黏附信号的展示，为再生医学和体外形态发生和致癌建模提供了众多优势。在这里，我们报告了设计胶原模拟肽（CMPs）的情况，这些肽在金属离子刺激下组装成高度交联的 3-D 基质，并且可以用 His 标记的货物（如绿色荧光蛋白（GFP-His8）和人表皮生长因子（hEGF-His6）进行功能化。发现结合的 hEGF-His6 会逐渐从基质中释放出来，并在 EGF 依赖性细胞系 MCF10A 中诱导细胞增殖。在 CMP 的 N 端额外掺入细胞黏附序列（RGDS），会创建一个有利于基质包封的 MCF10A 细胞组织成球体结构的环境，从而模拟 ECM 环境。
