大鼠甲状腺结合球蛋白的微观异质性

The microheterogeneity of rat TBG.

作者信息

Vranckx R, Savu L, Nunez E A

机构信息

U224, INSERM, Faculté de Médecine Xavier Bichat, Laboratoire de Biochimie, Paris, France.

出版信息

FEBS Lett. 1989 Feb 27;244(2):343-6. doi: 10.1016/0014-5793(89)80559-9.

DOI:10.1016/0014-5793(89)80559-9

PMID:2493395

Abstract

Isoelectric focusing (IEF) of native sera from immature or adult rats and of purified or partially purified rat serum thyroid hormone-binding proteins, demonstrates that rat TBG is a microheterogeneous protein. Autoradiography and radioactivity scans of the IEF plates show that it consists of at least four main isoforms, with bands at pH 4.35, 4.45, 4.5 and 4.55. This pattern is remarkably similar to that reported for human TBG. This is the first demonstration of the polymorphism of this recently discovered major binding protein of the rat.

摘要

对未成熟或成年大鼠的天然血清以及纯化或部分纯化的大鼠血清甲状腺激素结合蛋白进行等电聚焦（IEF）分析，结果表明大鼠甲状腺激素结合球蛋白（TBG）是一种微不均一蛋白。IEF板的放射自显影和放射性扫描显示，它至少由四种主要的同工型组成，在pH 4.35、4.45、4.5和4.55处有条带。这种模式与报道的人类TBG模式非常相似。这是首次证明这种最近发现的大鼠主要结合蛋白的多态性。