Schwartz John C, Murtaugh Michael P
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN, 55108, USA,
Immunogenetics. 2014 Aug;66(7-8):507-11. doi: 10.1007/s00251-014-0785-2. Epub 2014 Jun 17.
Swine, unlike other artiodactyls, but similar to humans, utilize both lambda and kappa light chain isotypes almost equally in the generation of their antibody repertoire. The porcine antibody light chain loci have previously been characterized in a single Duroc sow in which was seen extensive allelic variation between light chain genes on homologous chromosomes. However, the extent of variation between individuals is completely unknown. Using deep sequencing of cDNA-derived amplicons from five pigs, we report the identification and characterization of an IGLV gene that is functional and highly expressed in some animals, yet completely absent in others. Our findings provide a possible rationale for the known individual-to-individual variation in antibody responses to vaccination, infectious challenge, and subsequent disease outcome.
与其他偶蹄目动物不同，猪与人类相似，在产生抗体库时几乎同等程度地利用λ和κ轻链同种型。猪抗体轻链基因座先前已在一头杜洛克母猪中得到表征，在该母猪中，同源染色体上的轻链基因之间存在广泛的等位基因变异。然而，个体之间的变异程度完全未知。通过对五头猪的cDNA衍生扩增子进行深度测序，我们报告了一个IGLV基因的鉴定和表征，该基因在一些动物中具有功能且高度表达，但在其他动物中则完全缺失。我们的研究结果为已知的个体对疫苗接种、感染性挑战及后续疾病结果的抗体反应差异提供了一个可能的解释。
