Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 106, Taiwan.
Department of Chemical Engineering, Widya Mandala Catholic University, Kalijudan 37, Surabaya 60114, Indonesia.
Bioresour Technol. 2014 Aug;166:429-34. doi: 10.1016/j.biortech.2014.05.079. Epub 2014 May 28.
This study aims to investigate the harvesting of microalgae by dispersed air flotation (DiAF) using natural biosurfactant saponin as the collector and chitosan as the flocculant. Two types of microalgae, Chlorella vulgaris and Scenedesmus obliquus, were used in this study. It was observed that saponin was a good frother, but not an effective collector when used alone for flotation separation of algae. However, with the pre-flocculation of 5 mg/L of chitosan, separation efficiency of >93% microalgae cells was found at 20 mg/L of saponin. Removal efficiency of >54.4% and >73.0% was found for polysaccharide and protein, respectively at 20 mg/L of saponin and chitosan each. Experimental results show that DiAF using saponin and chitosan is effective for separation of microalgae, and algogenic organic matter (AOM). It can potentially be applied in the integrated microalgae-based biorefinery.
本研究旨在探讨利用天然生物表面活性剂皂素作为捕收剂和壳聚糖作为絮凝剂通过分散空气浮选（DiAF）来收获微藻。本研究使用了两种微藻，普通小球藻和斜生栅藻。研究表明，皂素是一种良好的起泡剂，但单独用作浮选分离藻类时效果不佳。然而，在用 5 mg/L 的壳聚糖进行预絮凝后，在 20 mg/L 的皂素下发现微藻细胞的分离效率>93%。在 20 mg/L 的皂素和壳聚糖下，多糖和蛋白质的去除效率分别>54.4%和>73.0%。实验结果表明，使用皂素和壳聚糖的 DiAF 对微藻和藻类源有机物（AOM）的分离是有效的。它可能有潜力应用于基于微藻的综合生物炼制厂。
