Machinaga Akihito, Takase-Yoden Sayaka
Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236, Tangi-machi, Hachioji-shi, Tokyo, 192-8577, Japan.
Microbiol Immunol. 2014 Aug;58(8):474-82. doi: 10.1111/1348-0421.12170.
As splicing was previously found to be important for increasing Friend murine leukemia virus env-mRNA stability and translation, we investigated whether splicing of env-mRNA affected the poly(A) tail length using env expression vectors that yielded unspliced or spliced env-mRNA. Incomplete polyadenylation was detected in a fraction of the unspliced env-mRNA products in an env gene-dependent manner, showing that splicing of Friend murine leukemia virus plays an important role in the efficiency of complete polyadenylation of env-mRNA. These results suggested that the promotion of complete polyadenylation of env-mRNA by splicing might partially explain up-regulation of Env protein expression as a result of splicing.
由于之前发现剪接对于提高弗氏鼠白血病病毒env - mRNA的稳定性和翻译很重要，我们使用产生未剪接或剪接的env - mRNA的env表达载体，研究了env - mRNA的剪接是否会影响聚腺苷酸尾长度。在一部分未剪接的env - mRNA产物中以env基因依赖的方式检测到不完全聚腺苷酸化，这表明弗氏鼠白血病病毒的剪接在env - mRNA完全聚腺苷酸化的效率中起重要作用。这些结果表明，剪接对env - mRNA完全聚腺苷酸化的促进作用可能部分解释了剪接导致Env蛋白表达上调的原因。
