Rafayelyan S, Radlanski R J, Minden K, Pischon N, Jost-Brinkmann P-G, Präger T M
Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
J Oral Pathol Med. 2015 Jan;44(1):67-74. doi: 10.1111/jop.12201. Epub 2014 Jun 16.
Juvenile idiopathic arthritis (JIA) of the temporomandibular joint (TMJ) can cause severe growth disturbances of the craniomandibular system. Antigen-induced arthritis (AIA) of the rabbit TMJ is simulating the inflammatory process of the TMJ in JIA. The aim of this study was to investigate the effect of a systemic administration of the tumor necrosis factor-alpha (TNF-α) antagonist etanercept on AIA in rabbits by means of three different histological staining methods.
After sensitization, a bilateral arthritis of the TMJ was induced and maintained by repeated intra-articular administrations of ovalbumin in 12 New Zealand white rabbits aged 10 weeks. From the 13th week of age, 6 of the 12 rabbits received weekly subcutaneous injections of etanercept, and the other 6 animals remained without therapy. Another 6 animals served as controls, receiving no treatment or intra-articular injections at all. After euthanasia at the age of 22 weeks, all TMJs were retrieved en bloc. Sagittal sections were cut and stained with hematoxylin-eosin (H-E), Safranin-O for the evaluation of the Mankin score, and tartrate-resistant acid phosphatase (TRAP).
In the arthritis group, a chronic inflammation with degeneration of the articular cartilage was visible. In the etanercept group, the signs of cartilage degeneration were significantly reduced but present. In contrast, the joints in the control group were inconspicuous. A strong correlation between the Mankin score and TRAP-positive cells could be found.
Antigen-induced arthritis causes severe damage in the TMJ of young rabbits. An improvement seems to be achievable by a systemic administration of etanercept.
颞下颌关节（TMJ）的幼年特发性关节炎（JIA）可导致颅下颌系统严重的生长紊乱。兔TMJ的抗原诱导性关节炎（AIA）模拟了JIA中TMJ的炎症过程。本研究的目的是通过三种不同的组织学染色方法，研究全身给予肿瘤坏死因子-α（TNF-α）拮抗剂依那西普对兔AIA的影响。
在致敏后，通过对12只10周龄的新西兰白兔反复关节内注射卵清蛋白，诱导并维持TMJ的双侧关节炎。从13周龄开始，12只兔子中的6只每周皮下注射依那西普，另外6只动物不接受治疗。另有6只动物作为对照，完全不接受治疗或关节内注射。在22周龄安乐死后，将所有TMJ整块取出。切取矢状切片，用苏木精-伊红（H-E）染色、番红O染色以评估曼金评分，并用抗酒石酸酸性磷酸酶（TRAP）染色。
在关节炎组中，可见关节软骨退变的慢性炎症。在依那西普组中，软骨退变的迹象明显减轻但仍存在。相比之下，对照组的关节不明显。曼金评分与TRAP阳性细胞之间存在很强的相关性。
抗原诱导性关节炎会对幼兔的TMJ造成严重损害。全身给予依那西普似乎可以实现病情改善。
