[血管内皮细胞对牙周膜细胞和牙龈成纤维细胞迁移的影响]

[The effect of vascular endothelial cells on the migration of periodontal ligament cells and gingival fibroblasts].

作者信息

Din Jin, Yin Yuan-zheng, Huang Jia-ni, Hu Cong-jiao

机构信息

Department of Periodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:

出版信息

Shanghai Kou Qiang Yi Xue. 2014 Apr;23(2):172-8.

PMID:24935838

Abstract

PURPOSE

To investigate the effect of human vascular endothelial cells (HUVEC) on the migration of human periodontal ligament cells (HPDLC) and human gingival fibroblasts (HGF) through a model mimicking periodontal wound healing or regeneration.

METHODS

HUVEC, HPDLC and HGF were co-cultured using a Transwell system, while HPDLC or HGF was cultured independently as control. Chemotaxis assay was performed using cell culture inserts (0.8μm pore size) to assess the effect of HUVEC on HPDLC and HGF vertical migration at 24h. A scratch wound assay was performed to assess the effect of HUVEC on HPDLC and HGF horizontal migration at 0 h, 8 h, 16 h and 24 h. A round glass sheet assay was performed to assess the effect of HUVEC on HPDLC and HGF wound healing ability at 1d, 4d and 7d. The data was processed with SPSS 13.0 software package.

RESULTS

At the round glass sheet assay, while HUVEC was present, the integrated option density (IOD) of HPDLCs and HGFs were significantly higher than each of the single culture (P<0.01). Results of chemotaxis assay and scratch wound assay showed that the cell number of HPDLCs and HGFs were significantly higher than each of the single culture while HUVEC was present (P<0.01), and the number of PHDLCs was significantly higher than HGFs on chemotaxis assay (P<0.01) but the result of scratch wound assay was on the contrary (P<0.01).

CONCLUSIONS

The presence of HUVEC can promote migration of periodontal cells, and the effect is more prominent on HPDLC than on HGF in vertical migration and wound healing, while horizontal migration is more prominent on HGFs as contrast.

摘要

目的

通过模拟牙周伤口愈合或再生的模型，研究人血管内皮细胞（HUVEC）对人牙周膜细胞（HPDLC）和人牙龈成纤维细胞（HGF）迁移的影响。

方法

使用Transwell系统将HUVEC、HPDLC和HGF共培养，而将HPDLC或HGF单独培养作为对照。使用细胞培养插入物（孔径0.8μm）进行趋化性测定，以评估24小时时HUVEC对HPDLC和HGF垂直迁移的影响。进行划痕试验以评估0小时、8小时、16小时和24小时时HUVEC对HPDLC和HGF水平迁移的影响。进行圆形玻璃板试验以评估1天、4天和7天时HUVEC对HPDLC和HGF伤口愈合能力的影响。数据用SPSS 13.0软件包处理。

结果

在圆形玻璃板试验中，当存在HUVEC时，HPDLC和HGF的积分光密度（IOD）显著高于各自的单培养（P<0.01）。趋化性测定和划痕试验结果表明，当存在HUVEC时，HPDLC和HGF的细胞数量显著高于各自的单培养（P<0.01），并且在趋化性测定中PHDLC的数量显著高于HGF（P<0.01），但划痕试验结果相反（P<0.01）。