克氏锥虫在盖氏梅氏锥蝽和斯氏梅氏锥蝽中的感染：野外若虫取食的影响。

Trypanosoma cruzi infection in Mepraia gajardoi and Mepraia spinolai: the effect of feeding nymphs from the field.

作者信息

Egaña Camila, Vergara Fernanda, Campos Ricardo, Ortiz Sylvia, Botto-Mahan Carezza, Solari Aldo

机构信息

Programa de Biología Celular y Molecular, Instituto de ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.

Programa de Biología Celular y Molecular, Instituto de ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

出版信息

Am J Trop Med Hyg. 2014 Sep;91(3):534-6. doi: 10.4269/ajtmh.13-0721. Epub 2014 Jun 16.

DOI:10.4269/ajtmh.13-0721

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4155554/

Abstract

We evaluated Trypanosoma cruzi infection rates by means of minicircle DNA-based polymerase chain reactions (PCRs) in 70 starved Mepraia gajardoi from northern Chile and 65 M. spinolai from central Chile after feeding. Immediately after collection in the field, 20% of M. gajardoi were found infected; after feeding, 67% of the uninfected were infected. One group of M. spinolai seemed to be completely uninfected, but after the first and second feedings, 62% and 59% were positive, respectively.

摘要

我们通过基于微小环DNA的聚合酶链反应（PCR）评估了来自智利北部的70只饥饿的盖氏梅氏锥蝽和来自智利中部的65只饱腹的斯氏梅氏锥蝽的克氏锥虫感染率。在野外采集后立即检测发现，20%的盖氏梅氏锥蝽已被感染；喂食后，67%未感染的锥蝽被感染。一组斯氏梅氏锥蝽似乎完全未被感染，但在第一次和第二次喂食后，阳性率分别为62%和59%。

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