照射前及SDF-1抑制对在不同基质床中生长的小鼠星形细胞瘤细胞进展的影响。
Effects of pre-irradiation and SDF-1 suppression on the progression of murine astrocytoma cells grown in different stromal beds.
作者信息
Yu Ching-Fang, Lin Chi-Min, Wang Shu-Chi, Chen Fang-Hsin, Hong Ji-Hong, Tsai Chien-Sheng, Yang Ying-Chieh, Chiang Chi-Shiun
机构信息
Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University , Hsinchu , Taiwan.
出版信息
Int J Radiat Biol. 2014 Dec;90(12):1162-8. doi: 10.3109/09553002.2014.930539. Epub 2014 Jul 7.
PURPOSE
To examine whether brain tumors grown in pre-irradiated (PreIR) thigh have a similar tumor bed effect (TBE) as in PreIR brain tissue.
MATERIAL AND METHODS
Tumor growth delay and immunohistochemical (IHC) staining for CD31, an endothelial surface marker, and PIMO, a hypoxia marker, were used to study the TBE of a murine astrocytoma, ALTS1C1, or a stromal-derived factor-1 (SDF-1) gene-silenced astrocytoma, ALTS1C1-SDFkd, growing in different PreIR stroma beds.
RESULTS
ALTS1C1 tumors growing in both PreIR brain and PreIR thigh had reduced microvascular density (MVD) and more chronic hypoxia, but tumor growth delay was only seen in PreIR brain tissue. In contrast, ALTS1C1-SDFkd tumors showed tumor growth delay in PreIR thigh, with little effect in PreIR brain tissue.
CONCLUSIONS
This study cautions that both the tumor and the nature of the PreIR stromal bed are important when using pre-irradiation as a model of recurrent brain tumors after radiation therapy.
目的
研究在预先照射（PreIR）的大腿中生长的脑肿瘤是否具有与在PreIR脑组织中相似的肿瘤床效应（TBE）。
材料与方法
使用肿瘤生长延迟以及针对内皮表面标志物CD31和缺氧标志物PIMO的免疫组织化学（IHC）染色，来研究在不同PreIR基质床中生长的小鼠星形细胞瘤ALTS1C1或基质衍生因子-1（SDF-1）基因沉默的星形细胞瘤ALTS1C1-SDFkd的TBE。
结果
在PreIR脑和PreIR大腿中生长的ALTS1C1肿瘤微血管密度（MVD）降低且慢性缺氧更严重，但仅在PreIR脑组织中观察到肿瘤生长延迟。相比之下，ALTS1C1-SDFkd肿瘤在PreIR大腿中显示出肿瘤生长延迟，而在PreIR脑组织中影响较小。
结论
本研究警示，将预先照射作为放射治疗后复发性脑肿瘤的模型时，肿瘤以及PreIR基质床的性质均很重要。
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