Laboratory of Genome Variations and Precision Bio-Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Laboratory of Genome Variations and Precision Bio-Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):692-6. doi: 10.1016/j.bbrc.2014.06.033. Epub 2014 Jun 14.
Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in an AR dependent manner. Furthermore, SET8 is crucial for the transcription activation of PSA. Co-immunoprecipitation analyses demonstrate that SET8 interacts with AR. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification.
组蛋白在不同赖氨酸残基上的甲基化状态在转录调控中起着重要作用。H4K20 单甲基化（H4K20me1）对雄激素受体（AR）介导的基因转录的影响尚不清楚。在这里，我们显示 AR 激动剂以 AR 依赖的方式刺激 PSA 等 AR 靶基因启动子处 H4K20me1 和 SET8 的富集。此外，SET8 对于 PSA 的转录激活至关重要。免疫共沉淀分析表明 SET8 与 AR 相互作用。因此，我们得出结论，SET8 参与 AR 介导的转录激活，可能通过其与 AR 和 H4K20me1 修饰的相互作用。
