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临武鸭线粒体基因组的基因组织与完整序列

Gene organization and complete sequence of the mitochondrial genome of Linwu mallard.

作者信息

Tian Ke-Xiong, Liu Li-Li, Yu Qi-Fang, He Shao-Ping, He Jian-Hua

机构信息

a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.

b College of Life Science, Hunan University of Science and Technology , Xiangtan , P.R. China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):910-1. doi: 10.3109/19401736.2014.926481. Epub 2014 Jun 18.

Abstract

Linwu mallard is an excellent native breeds from Hunan province in China. This is the first study to determine the complete mitochondrial genome sequence of L. mallard using PCR-based amplification and Sanger sequencing. The characteristic of the entire mitochondrial genome was analyzed in detail, with the base composition of 29.19% A, 22.19% T, 32.83% C, 15.79% G in the L. mallard (16,605 bp in length). It contained 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and a major non-coding control region (D-loop region). The complete mitochondrial genome sequence of L. mallard will be useful for the phylogenetics of poultry, and be available as basic data for the genetics and breeding.

摘要

临武鸭是中国湖南省优良的地方品种。这是首次利用基于PCR的扩增和桑格测序法确定临武鸭线粒体基因组全序列的研究。详细分析了整个线粒体基因组的特征,临武鸭线粒体基因组(长度为16,605 bp)的碱基组成为:A占29.19%、T占22.19%、C占32.83%、G占15.79%。它包含2个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和一个主要的非编码控制区(D-loop区)。临武鸭线粒体基因组全序列将有助于家禽系统发育研究,并可为遗传育种提供基础数据。

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