Li Chuanwu, Liang Zhiqiang, Li Hong, Yuan Xiping
a Fishery Environmental Monitoring Station, Fisheries Research Institute of Hunan province , Changsha , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):933-4. doi: 10.3109/19401736.2014.926492. Epub 2014 Jun 18.
The complete mitochondrial genome of Sinilabeo decorus tungting was determined in this study. The gene composition, arrangement and transcriptional orientation in S. decorus tungting mitogenome were identical to most vertebrates. Two start codon patterns (ATG and GTG) and three stop codon patterns (TAG, TAA and T) were found in protein-coding genes. Only the tRNA-Ser2 could not fold into a typical clover-leaf secondary structure for lacking the dihydrouridine arm. Sequences alignment results suggest that the complete mitogenome of S. decorus tungting is an efficient tool to check species identification by comparing different gene sequences.
本研究测定了墨头鱼属东洞墨头鱼的完整线粒体基因组。东洞墨头鱼线粒体基因组中的基因组成、排列和转录方向与大多数脊椎动物相同。在蛋白质编码基因中发现了两种起始密码子模式(ATG和GTG)和三种终止密码子模式(TAG、TAA和T)。只有tRNA-Ser2因缺乏二氢尿嘧啶臂而无法折叠成典型的三叶草二级结构。序列比对结果表明,通过比较不同的基因序列,东洞墨头鱼的完整线粒体基因组是检验物种鉴定的有效工具。