产气气杆菌62-1无细胞系统对赖氨酸：N6-羟基化作用的研究。
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1.
作者信息
Goh C J, Szczepan E W, Menhart N, Viswanatha T
机构信息
Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Waterloo, Canada.
出版信息
Biochim Biophys Acta. 1989 Mar 24;990(3):240-5. doi: 10.1016/s0304-4165(89)80040-6.
PMID:2493814
Abstract
Electron microscopic examination has revealed the vesicular nature of the membrane component, of the cell-free system of Aerobacter aerogenes 62-1, which catalyses lysine: N6-hydroxylation. Regardless of the orientation of the vesicles, N-hydroxylation process is still stimulated by pyruvate. Both pyruvate oxidation and lysine: N6-hydroxylation were inhibited by protonophores and Gramicidin S.
摘要
电子显微镜检查揭示了产气气杆菌62-1无细胞系统中催化赖氨酸：N6-羟基化的膜成分的囊泡性质。无论囊泡的取向如何，丙酮酸仍能刺激N-羟基化过程。质子载体和短杆菌肽S均可抑制丙酮酸氧化和赖氨酸：N6-羟基化。
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