Goh C J, Szczepan E W, Menhart N, Viswanatha T
Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Waterloo, Canada.
Biochim Biophys Acta. 1989 Mar 24;990(3):240-5. doi: 10.1016/s0304-4165(89)80040-6.
Electron microscopic examination has revealed the vesicular nature of the membrane component, of the cell-free system of Aerobacter aerogenes 62-1, which catalyses lysine: N6-hydroxylation. Regardless of the orientation of the vesicles, N-hydroxylation process is still stimulated by pyruvate. Both pyruvate oxidation and lysine: N6-hydroxylation were inhibited by protonophores and Gramicidin S.
电子显微镜检查揭示了产气气杆菌62-1无细胞系统中催化赖氨酸：N6-羟基化的膜成分的囊泡性质。无论囊泡的取向如何，丙酮酸仍能刺激N-羟基化过程。质子载体和短杆菌肽S均可抑制丙酮酸氧化和赖氨酸：N6-羟基化。
