用于木薯（大戟科）种质鉴定的反转录转座子间扩增多态性标记

Inter-retrotransposon-amplified polymorphism markers for germplasm characterization in Manihot esculenta (Euphorbiaceae).

作者信息

Oliveira-Silva A M, Silva G F, Dias M C, Clement C R, Sousa N R

机构信息

Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, Manaus, AM, Brasil.

Laboratório de Biologia Molecular, Embrapa Amazônia Ocidental, Manaus, AM, Brasil.

出版信息

Genet Mol Res. 2014 May 16;13(2):3800-4. doi: 10.4238/2014.May.16.3.

DOI:10.4238/2014.May.16.3

PMID:24938466

Abstract

Manioc, Manihot esculenta, is economically important in many tropical and subtropical countries. The genetic variability of the species has not been fully explored, and new information may help expand its use. Molecular markers based on retrotransposons have good potential for analysis of genetic diversity given their abundance in the genome. Eight long terminal repeat retrotransposons were selected for the development of inter-retrotransposon-amplified polymorphism markers. To test these primers, we analyzed 32 varieties from Anori, 30 from Manicoré and 10 Mandiocabas from the Manioc Germplasm Bank at Embrapa Western Amazonia. The six informative primer pairs yielded 20- 60 polymorphic bands, averaging 92% polymorphism (51.7-98.4) and 0.37 heterozygosity (0.17 to 0.40), with a Shannon information index of 0.54 (0.26-0.59). These markers can be used to explore the genetic diversity of manioc.

摘要

木薯（Manihot esculenta）在许多热带和亚热带国家具有重要的经济价值。该物种的遗传变异性尚未得到充分研究，新的信息可能有助于扩大其用途。基于反转录转座子的分子标记因其在基因组中的丰富性而具有分析遗传多样性的良好潜力。选择了八个长末端重复反转录转座子来开发反转录转座子间扩增多态性标记。为了测试这些引物，我们分析了来自巴西农牧业研究公司亚马逊西部地区木薯种质库的32个阿诺里品种、30个马尼科雷品种和10个曼迪奥卡巴斯品种。这六对信息性引物产生了20至60条多态性条带，平均多态性为92%（51.7 - 98.4），杂合度为0.37（0.17至0.40），香农信息指数为0.54（0.26 - 0.59）。这些标记可用于探索木薯的遗传多样性。