Anitha S, Monyo E S, Okori P
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), P.O. Box 1096, Lilongwe, Malawi,
Arch Virol. 2014 Nov;159(11):3059-62. doi: 10.1007/s00705-014-2139-7. Epub 2014 Jun 18.
Groundnut rosette disease (GRD) is the most devastating disease of groundnuts in sub-Saharan Africa. The disease is caused by synergistic interactions between viruses and virus-like pathogens: groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and a satellite RNA (satRNA). The multi-pathogenic nature of GRD requires efficient diagnostic systems for plant breeding and pathology work. Currently, TAS-ELISA and RT-PCR are used to detect all three pathogens. This approach is time-consuming, expensive and not easily amenable to high throughput. A multiplex PCR-based approach was developed to detect all three pathogens at once, reducing diagnostics costs and time by two thirds. The technique is highly robust and amenable to high throughput, with sensitivity and specificity values of 88 % and 100 %, respectively. The positive predictive value for the technique is 100 %, and the negative predictive value is 90.6 %.
花生丛簇病（GRD）是撒哈拉以南非洲地区花生最具毁灭性的病害。该病害由病毒与类病毒病原体之间的协同相互作用引起：花生丛簇辅助病毒（GRAV）、花生丛簇病毒（GRV）和一种卫星RNA（satRNA）。GRD的多病原性质要求有高效的诊断系统用于植物育种和病理学研究。目前，TAS-ELISA和RT-PCR用于检测所有三种病原体。这种方法耗时、昂贵且不易于高通量操作。一种基于多重PCR的方法被开发出来，可一次性检测所有三种病原体，将诊断成本和时间降低了三分之二。该技术高度稳健且适合高通量操作，灵敏度和特异性值分别为88%和100%。该技术的阳性预测值为100%，阴性预测值为90.6%。
